| DB ID | MyCo_3904 |
| Title | Interleukin-12 neutralization alters lung inflammation and leukocyte expression of CD80, CD86, and major histocompatibility complex class II in mice infected with Histoplasma capsulatum |
| Year | 2000 |
| PMID | 10722603 |
| Fungal Diseases involved | Histoplasma capsulatum infection |
| Associated Medical Condition | None |
| Genus | Histoplasma |
| Species | capsulatum |
| Organism | Histoplasma capsulatum |
| Ethical Statement | None |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Biopsy |
| Sample source | Extracted cell supernatant |
| Host Group | Animal |
| Host Common name | Mice |
| Host Scientific name | Mus musculus |
| Biomarker Name | CD86 |
| Biomarker Full Name | CD86 |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | USA |
| Cohort | Male C57BL/6 mice were purchased from Jackson Laboratory (Bar Harbor, Maine) and maintained in the animal facility at the University of Cin- cinnati. All experiments used animals that were 6 to 12 weeks of age. Control and infected mice were housed in laminar flow units. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Histoplasma capsulatum is an intracellular pathogenic fun- gus that is responsible for mild disease in immunocompetent hosts and a progressive and fatal disease if untreated in im- munocompromised hosts. The initial site of infection is the lung, where yeast cells, produced from inhaled microconidia, are ingested by alveolar macrophages (M ) via an interaction between the CD11/CD18 family of adhesion molecules and yeast cell wall components. Phagocytosis of yeast cells by M results in a permissive environment for survival and rep- lication of yeasts. Resistance to H. capsulatum infection in mammals is primarily dependent on a cellular immune re- sponse mediated by T cells and phagocytes. Resolution of infection in mice requires the production of cytokines, espe- cially gamma interferon (IFN- ), and release of this cytokine by NK and T cells is dependent on the pathogen- induced release of the monokine interleukin-12 (IL-12). H. capsulatum infection of mice with a genetic absence of IFN- or those given antibodies (Ab) to IL-12 results in an uncontrollable and fatal fungal burden. IL-12 release is necessary for M to kill yeasts before day 5 of infection, since animals depleted of IL-12 beyond this point survive the infection. |
| Technique | Immunological assay |
| Analysis Method | Immunoassay |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | ELISA,Immunoassay |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
