| DB ID | MyCo_3804 |
| Title | Early and Non-invasive Diagnosis of Aspergillosis Revealed by Infection Kinetics Monitored in a Rat Model |
| Year | 2018 |
| PMID | 30349512 |
| Fungal Diseases involved | Aspergillosis |
| Associated Medical Condition | None |
| Genus | Aspergillus |
| Species | fumigatus |
| Organism | Aspergillus fumigatus |
| Ethical Statement | All animal experiments were conducted in accordance with the regulations and guidelines of the Czech Animal Protection Act (No. 246/1992) and with the approval of the Ministry of Education, Youth and Sports of the Czech Republic (MSMT-21275/2016-2) and the institutional Animal Welfare Committee of the Faculty of Medicine and Dentistry of Palacký University in Olomouc. The care of research staff conformed to the general guidelines for the protection of the European Community (86/609/EEC, 200/54/EC 16) and included the use of respiratory protective equipment with the standard FFP2 equivalent to an N95 HEPA filter. |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Body fluid |
| Sample source | Urine |
| Host Group | Animal |
| Host Common name | Mice |
| Host Scientific name | Mus musculus |
| Biomarker Name | TAFC |
| Biomarker Full Name | Triacetylfusarinine C |
| Biomarker Type | Diagnostic |
| Biomolecule | Metabolite |
| Geographical Location | Czechia |
| Cohort | Using a model of experimental aspergillosis in immunocompromised Lewis rats, the fungal siderophores ferricrocin (FC) and triacetylfusarinine C (TAFC) were monitored in rat urine before and after lung inoculation with A. fumigatus conidia. Molecular biomarkers in high dose (HD) and low-dose (LD) infection models were separated using high performance liquid chromatography (HPLC) and were detected by mass spectrometry (MS). In the current work, we corroborated the in vivo MS infection kinetics data with micro-positron emission. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Aspergillus fumigatus is a ubiquitous saprophytic airborne fungus that causes life-threatening pulmonary infections in immunocompromised hosts. A. fumigatus has also recently emerged as an important pathogen in immunocompetent patients in intensive care units. In 2018, David W. Denning reported that estimated 14,700,000 cases of aspergillosis occur resulting in 1,010,000 deaths every year. On an annual basis, mainly invasive pulmonary aspergillosis (IPA; 200,000–400,000), chronic pulmonary aspergillosis (CPA; 1.5–3 M) and allergic bronchopulmonary aspergillosis (ABPA; 6–20 M) account for these aspergillosis cases, with mortality rates of 30–85%, 45% within 5 years, and <1%, respectively, for the treated cases of these three prevailing aspergillosis forms. These rapidly growing incidence numbers together with emerging pan-azole-resistant Aspergillus strains define aspergillosis as an important infectious disease for which no vaccine is yet available. |
| Technique | Liquid chromatography |
| Analysis Method | High performance liquid chromatography (HPLC) mediated Mass spectrometry (MS) |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | High performance liquid chromatography (HPLC) mediated Mass spectrometry (MS), µPET/CT imaging |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
