| DB ID | MyCo_3665 |
| Title | Role of Pneumocystis jirovecii infection in chronic obstructive pulmonary disease progression in an immunosuppressed rat Pneumocystis pneumonia model |
| Year | 2020 |
| PMID | 32256801 |
| Fungal Diseases involved | Chronic Obstructive Pulmonary Disease |
| Associated Medical Condition | None |
| Genus | Pneumocystis |
| Species | jirovecii |
| Organism | Pneumocystis jirovecii |
| Ethical Statement | The protocol for animal experimentation was approved by the Experimental Animal Care and Ethics Committee of China Medical University (Liaoning, China). |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Macromolecule |
| Sample source | Extracted Protein |
| Host Group | Animal |
| Host Common name | Rat |
| Host Scientific name | Rattus norvegicus |
| Biomarker Name | MMP-2 |
| Biomarker Full Name | matrix metalloprotease-2 |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | China |
| Cohort | A total of 20 female Wistar rats (mean weight, 150±20 g; age, 5 7 weeks) were purchased from the Department of Experimental Animal Center, China Medical University. Rats were randomly divided into two groups: A control group and a PCP group with ten rats per group. All rats were housed in a temperature (20 25˚C) and humidity (53 58%) controlled facility with a 12 h light/dark cycle with food and water provided ad libitum. The antibiotic oxytetracycline (1 g/l; Yichuang Pharmaceutical Co., Ltd.) was added to the drinking water to prevent bacterial infections. The immune response of the PCP group rats was suppressed by intraperitoneal dexamethasone sodium phosphate injections (3 mg per rat; Rongsheng Pharmaceutical Co., Ltd) twice per week, for 8 successive weeks. The control group was injected with physiological saline. The rats were weighed once a week. Fur color and thickness, activity, breathing, death rate, and other symptoms and signs of respiratory distress were monitored throughout the study. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Chronic obstructive pulmonary disease (COPD) remains one of the major causes of morbidity and mortality worldwide and is a large global public health burden. Cigarette smoking is the major risk factor for COPD with long term exposure to ciga¬rette smoke the most common method for establishing COPD in animal models. However, there is increasing attention on the role of infection in the development of COPD, with the opportunistic pathogen Pneumocystis jirovecii (P. jirovecii) likely to be a key contributor |
| Technique | Electrophoresis |
| Analysis Method | gelatin zymography |
| ELISA kits | ELISA kits (Cusabio Biotech Co., Ltd.): MMP‑2 ELISA kit (cat. no. CSB‑E07411r), MMP-8 ELISA kit (cat. no. CSB‑E07406r), MMP-9 ELISA Kit (cat. no. CSB‑E08008r), MMP-12 ELISA kit (cat. no. CSB‑EL014659RA), HSP-27 ELISA Kit (cat. no. CSB‑E09240r) |
| Assay Data | None |
| Validation Techniques used | ELISA, qRT-PCR, Western Blot |
| Up Regulation Down Regulation | Increase |
| Sequence Data | None |
| External Link | None |
