| DB ID | MyCo_3505 |
| Title | Deficient Phagocytosis in Circulating Monocytes from Patients with COVID-19-Associated Mucormycosis |
| Year | 2023 |
| PMID | 37052373 |
| Fungal Diseases involved | Mucormycosis |
| Associated Medical Condition | Covid-19 |
| Genus | None |
| Species | None |
| Organism | None |
| Ethical Statement | The study was approved by the Human Ethics Committee of Medical College, Kolkata (No. MC/KOL/IEC/NON-SPON/1102/06/2021). |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Body fluid |
| Sample source | Plasma |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | RANTES |
| Biomarker Full Name | RANTES |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | India |
| Cohort | A small cohort of Indian patients suffering from CAM (N = 9) as well as COVID-19 patients with no evidence of mucormycosis (N = 5) were recruited in the study. Venous blood was collected from the patients as well as from healthy volunteers (N = 8). Peripheral blood mononuclear cells and plasma were isolated. Plasma samples were used to measure a panel of 48 cytokines. CD141 monocytes were isolated and used for a ow cytometric phagocytosis assay as well as a global transcriptome analysis via RNA-sequencing. |
| Cohort No. | 14 Patients and 8 control |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Asurge in cases of rhino-orbital mucormycosis, mainly caused by Rhizopus species, in patients suffering from severe coronavirus disease 2019 (COVID-19) was reported in India as well as in other parts of the world. Diabetes mellitus, corticosteroid pharmaco- therapy, environmental exposure, and immune dysregulation are proposed as major predis- posing factors for the development of COVID-19 associated mucormycosis (CAM). Speci c immune mechanisms linked to the susceptibility to CAM remain largely unexplored. The inhibition of the growth of fungal conidia, following their uptake by tissue-resi- dent and circulating phagocytes, is a crucial antifungal host defense mechanism against mucorales. Accordingly, defects in the numbers or functions of phagocytes markedly increase the susceptibility to mucormycosis. |
| Technique | Analytic |
| Analysis Method | Fow cytometric phagocytosis assay based global transcriptome analysis |
| ELISA kits | phagocytosis assay Kit (Bio-Plex Pro 48-Plex Assay, Bio-Rad) |
| Assay Data | None |
| Validation Techniques used | Fow cytometric phagocytosis assay based global transcriptome analysis |
| Up Regulation Down Regulation | Decrease |
| Sequence Data | None |
| External Link | None |
