| DB ID | MyCo_3500 |
| Title | IL-9 Deficiency Promotes Pulmonary Th17 Response in Murine Model of Pneumocystis Infection |
| Year | 2018 |
| PMID | 29887863 |
| Fungal Diseases involved | Pneumocystis pneumonia |
| Associated Medical Condition | None |
| Genus | None |
| Species | None |
| Organism | None |
| Ethical Statement | All animal work was conformed to the Ethics Committee of Capital Medical University. |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Body fluid |
| Sample source | Bronchoalveolar lavage fluid (BALF) |
| Host Group | Animal |
| Host Common name | Mice |
| Host Scientific name | Mus musculus |
| Biomarker Name | IL-9 |
| Biomarker Full Name | Interleukin-9 |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | China |
| Cohort | Healthy male BALB/c and severe combined immunodeficiency (Scid) mice were purchased at 6–7 weeks of age from Vital River Lab Animal Co., Ltd. (Beijing, China), weighting 20–22 g. IL-9‒/‒ mice (BALB/c background) were kindly provided by Professor Andrew McKenzie of MRC Laboratory of Molecular Biology, Cambridge, UK. IL-17-deficient (IL-17‒/‒) mice (C57BL/6 background) were generated as previously described and provided by Dr. Iwakura of University of Tokyo. Animals were housed in specific pathogen-free conditions. All of the procedures were approved by the Capital Medical University Animal Care and Use Committee. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Pneumocystis is an opportunistic fungal pathogen which causes often fatal pneumonia in immunocompromised individuals. The incidence and mortality of Pneumocystis pneumonia (PCP) in HIV patients have decreased continuously due to highly activate antiretroviral therapy; however, PCP rate is increasing in non-HIV compromised patients and the reported mortality of non-HIV PCP patients is as high as 62% in intensive care unit. Host immunity defense mechanisms against Pneumocystis organism remains poorly understood. The onset of PCP is typically related to CD4+ T cell count less than 200 cells/μL, which indicates the key role of this lymphocyte subset in defense against Pneumocystis infection. |
| Technique | Analytic |
| Analysis Method | Flow Cytometry Analysis |
| ELISA kits | ELISA kit eBioscience (San Diego, CA, USA) |
| Assay Data | None |
| Validation Techniques used | ELISA, RT-PCR, Flow Cytometry |
| Up Regulation Down Regulation | Increase |
| Sequence Data | None |
| External Link | None |
