| DB ID | MyCo_3444 |
| Title | Phenotypic and functional characterization of pulmonary macrophages subpopulations after intratracheal injection of Paracoccidioides brasiliensis cell wall components |
| Year | 2010 |
| PMID | 21257226 |
| Fungal Diseases involved | Paracoccidioidomycosis |
| Associated Medical Condition | None |
| Genus | Paracoccidioides |
| Species | brasiliensis |
| Organism | Paracoccidioides brasiliensis |
| Ethical Statement | All animal manipulations were done according to preapproved institutional guidelines from Ethics Committee from University of São Paulo. |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Body fluid |
| Sample source | Bronchoalveolar lavage fluid (BALF) |
| Host Group | Animal |
| Host Common name | Mice |
| Host Scientific name | Mus musculus |
| Biomarker Name | TNF-α |
| Biomarker Full Name | Tumor Necrosis Factor alpha |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | Brazil |
| Cohort | Male isogenic BALB/c and C57Bl/6 at a body weight of about 20 g and at an age of 6–8 weeks were purchased through Univer- sity of São Paulo animal facility and were housed in wire cages at room temperatures in a 12–12 h light–dark cycle and given food and water ad libitum. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | The experimental paracoccidioidomycosis (PCMEx) caused by the dimorphic fungus Paracoccidioides brasiliensis is an inter-esting model to better understand inflammatory patterns of pulmonary macrophages (PM) populations. The human paracoccidioidomycosis (PCMHu) is characterized as chronic and progressive granulomatous disease with several regulatory disturbances of the immunity that seems to be ineffective against the fungus. The lungs are primary targets in human paracoccidioidomycosis; however, the potential role of PM in PCMHu is still unclear. |
| Technique | Analytic |
| Analysis Method | Flow Cytometry Analysis |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | Flow Cytometry Analysis |
| Up Regulation Down Regulation | Increase |
| Sequence Data | None |
| External Link | None |
