| DB ID | MyCo_3438 |
| Title | Analysis of invariant natural killer T cells in human paracoccidioidomycosis |
| Year | 2011 |
| PMID | 21805204 |
| Fungal Diseases involved | Paracoccidioidomycosis |
| Associated Medical Condition | None |
| Genus | Paracoccidioides |
| Species | brasiliensis |
| Organism | Paracoccidioides brasiliensis |
| Ethical Statement | The study protocol was approved by the ethics committee of the Hospital das Clı ´nicas da Universidade de Sa ˜o Paulo. |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Body fluid |
| Sample source | Blood |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | iNKT cells |
| Biomarker Full Name | Invariant NKT cells |
| Biomarker Type | Negative |
| Biomolecule | Protein |
| Geographical Location | Brazil |
| Cohort | Three groups were enrolled in this study: (1) 7 healthy individuals (29–53 years old), selected from the records of Hospital das Clı ´nicas da Universidade de Sa ˜o Paulo, who have had PCM in the past, had been treated for at least 2 years until considered cured, and remained healthy for at least the last 5 years after the completion of treatment (healthy cured [HC] group). They presented no sequels, had serological data indicating none reactivation of PCM, and had no sign or symptoms of any other debilitating disease at the moment of the study. (2) 5 healthy individuals who never had PCM or any other debilitating disease in the past (20–44 years old) and were non-reactors to the skin test with paracoccidioidin (healthy non-infected [HNI] group). (3) 5 paracoccidioidin-reactive individuals ( [10 mm indu- ration after 48 h, age range: 20–42 years), recruited from an endemic area located at the southwest of Brazil (Prata ˆnea, Sa ˜o Paulo state, Brazil) (healthy infected [HI] group). |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Paracoccidioidomycosis (PCM) is the most prevalent systemic mycosis in Latin America and still the leading cause of death among the mycoses. Its causative agent, Paracoccidioides brasiliensis, is a thermo-dimorphic fungus that grows as a yeast-like structure in the host tissue and as mycelium in the environment. The infection is acquired by the inhalation of conidia produced by mycelia present in the soil under certain conditions. These conidia reach the lungs where they differentiate into the yeast form establishing the infectious process. |
| Technique | Analytic |
| Analysis Method | Flow Cytometry Analysis |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | Flow Cytometry Analysis |
| Up Regulation Down Regulation | Negative |
| Sequence Data | None |
| External Link | None |
