| DB ID | MyCo_3387 |
| Title | Immunomodulation with CD40 stimulation and interleukin-2 protects mice from disseminated cryptococcosis |
| Year | 2006 |
| PMID | 16552046 |
| Fungal Diseases involved | Cryptococcosis |
| Associated Medical Condition | None |
| Genus | Cryptococcus |
| Species | neoformans |
| Organism | Cryptococcus neoformans |
| Ethical Statement | All studies were approved by the Institutional Animal Care and Use Committee, University of Nevada, Reno, 1664 N. Virginia St., Reno, NV 89557, USA. |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Body fluid |
| Sample source | Blood |
| Host Group | Animal |
| Host Common name | Mice |
| Host Scientific name | Mus musculus |
| Biomarker Name | anti-CD40 |
| Biomarker Full Name | anti-CD40 |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | USA |
| Cohort | C57BL/6 mice were obtained from the Charles River Laboratory of the National Cancer Institute (Frederick, MD). B6.129S7-Ifngtm1Ts/J (IFN- knockout [GKO]) and C57BL/6J wild-type (WT) control mice were purchased from The Jackson Laboratory (Bar Harbor, ME). |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Cryptococcus neoformans is an encapsulated yeast that can cause significant disease in immunocompromised hosts. Al- though the pulmonary tract is considered to be the major infection route, meningitis and meningoencephalitis caused by dissemination of C. neoformans to the brain are the most common manifestations and the main reason for high mortality of cryptococcosis. In murine models, C. neoformans infection through the intravenous route has been used widely to study the pathology as well as treatment of disseminated cryptococcosis. |
| Technique | Analytic |
| Analysis Method | Flow Cytometry Analysis |
| ELISA kits | Quantikine M mouse IFNγ and TNF-α immunoassay kit (R&D Systems, Minneapolis, MN). |
| Assay Data | None |
| Validation Techniques used | Flow Cytometry Analysis, ELISA |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
