| DB ID | MyCo_3291 |
| Title | Quantification of A. fumigatus-specific CD154+ T-cells-preanalytic considerations |
| Year | 2016 |
| PMID | 27486216 |
| Fungal Diseases involved | Invasive aspergillosis |
| Associated Medical Condition | None |
| Genus | Aspergillus |
| Species | fumigatus |
| Organism | Aspergillus fumigatus |
| Ethical Statement | This study was approved by the Ethics Committee of the University of Wuerzburg. |
| Site of Infection | None |
| Opportunistic invasive | Invasive |
| Sample type | Body fluid |
| Sample source | Blood |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | CD154 |
| Biomarker Full Name | CD154 |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | Germany |
| Cohort | After obtaining written informed consent (approved by the Ethics Committee of the University of Wuerzburg), 18 ml of EDTA whole blood were collected from healthy volunteers. Peripheral blood mononuclear cells (PBMCs) were purified from 26 healthy donors (9 male, 17 female, mean age 23.7 years) by Ficoll gradient centrifugation. |
| Cohort No. | 26 |
| Age Group | 23.7 |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Invasive aspergillosis (IA) is a common opportunistic fungal infection in immunocompromised patients. Early and reliable diagnosis is pivotal in order to improve therapeutic out-comes. It has become clear that T-cell immunity significantly contributes to the defence against mould infections. Recently, it was shown that the quantification of fungus specific CD154+ T cells may provide a novel diagnostic assay for the rapid identification of invasive pulmonary mould infections. CD154 is a T-cell activation marker transiently expressed on CD4+ cells upon stimulation of their T-cell receptor. Different studies showed that ex vivo stimulation of mononuclear cells followed by flow cytometric quantification of CD154-positive cells is a sensitive and convenient approach for the assessment of antigen-specific T-cell response. |
| Technique | Analytic |
| Analysis Method | Flow Cytometry Analysis |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | Flow Cytometry Analysis |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
