| DB ID | MyCo_3288 |
| Title | Pyruvate kinase M2 in lung APCs regulates Alternaria-induced airway inflammation |
| Year | 2020 |
| PMID | 32747016 |
| Fungal Diseases involved | Alternaria alternata infection |
| Associated Medical Condition | Asthma |
| Genus | Alternaria |
| Species | alternata |
| Organism | Alternaria alternata |
| Ethical Statement | All animal procedures and experimental protocols were approved by the Auburn University Animal Care and Use Committee. |
| Site of Infection | Lungs |
| Opportunistic invasive | None |
| Sample type | Biopsy |
| Sample source | Extracted tissue |
| Host Group | Animal |
| Host Common name | Mice |
| Host Scientific name | Mus musculus |
| Biomarker Name | Hif-1α |
| Biomarker Full Name | Hypoxia-inducible factor −1α |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | Auburn, AL |
| Cohort | Six to eight weeks old naïve C57BL/6J and CD11c-DTR transgenic mice (004509) were purchased from Jackson Laboratory (Bar Harbor, MA). Mice were sensitized intranasally with single administration of Alternaria alternata or HDM extracts (50 μg in 40 μl of PBS). |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Fungal allergens are proven risk-factor for asthma severity. Sensitization to fungal allergens such as Alternaria alternata are associated with fatal asthma exacerbations and hospitalizations. Among various common atmospheric mold, Alternaria spores are highly prevalent in grain-growing areas during late summer and/or early autumn period and leads to increased incidents of asthma episodes. In clinic, 38.3% of asthmatic children are positive for Alternaria species and manifests symptom with recurrent wheezing and increased airway responsiveness to methacholine. Previous animal studies have demonstrated various mechanism for unravelling the intricacies of Alternaria -induced allergic asthma. An intrinsic protease activity of Alternaria act as effective adjuvant to drive prolonged Th2 type inflammation. Alternaria -mediated development of allergic asthma is driven by IL33-ST2 axis in the lung. As such, Alternaria -induced activation of protease activity receptor 2 (PAR-2) in airway epithelial cells is associated with the IL33 release and development of acute airway inflammation. |
| Technique | Analytic |
| Analysis Method | Flow Cytometry Analysis |
| ELISA kits | murine Duoset ELISA kit (R&D systems). |
| Assay Data | None |
| Validation Techniques used | ELISA, Immunofluorescence assay, Flow Cytometry Analysis, qTR-PCR, Seahorse XFp assays |
| Up Regulation Down Regulation | Increase |
| Sequence Data | None |
| External Link | None |
