| DB ID | MyCo_3285 |
| Title | In vitro and in vivo effect of PD-1/PD-L1 blockade on microglia/macrophage activation and T cell subset balance in cryptococcal meningitis |
| Year | 2017 |
| PMID | 29058791 |
| Fungal Diseases involved | Cryptococcal meningitis |
| Associated Medical Condition | None |
| Genus | Cryptococcus |
| Species | spp. |
| Organism | Cryptococcus spp. |
| Ethical Statement | All study participants enrolled in the study were provided with written informed consent documentation. All animals participating in the study were raised and treated in accordance with the Guide for the Care and Use of Laboratory Animals. All procedure and protocols of this study were performed with the approval of the Ethics Committee of the First Affiliated Hospital of Nanchang University. Approval from both the Institutional Review Board (IRB) as well as the Institutional Animal Care and Use Committee (IACUC) was obtained for the purposes of this study. |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Body fluid |
| Sample source | Blood |
| Host Group | Animal |
| Host Common name | Mice |
| Host Scientific name | Mus musculus |
| Biomarker Name | CM + AmB |
| Biomarker Full Name | Cryptococcal meningitis + amphotericin B |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | China |
| Cohort | The CM group consisted of 126 patients (75 males and 51 females) and aged between 14-77 years (mean age = 45.92 ± 14.54 years). The 126 patients had previously been pathologically diagnosed with CM at the First Affiliated Hospital of Nanchang University between March 2010 and December 2016. Seventy five C57BL/6 mice were grouped into the normal control, CM model, CM + AmB, sham and CM + PD-1 antibodies (Ab) groups. CD4+ and CD8+ T cells as well as microglia/macrophages were analyzed by means of flow cytometry. |
| Cohort No. | 126 |
| Age Group | None |
| P Value | p=0.0132 |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Crytococcosis is an opportunistic fungal disease, instigated by Cryptococcus neoformans or Cryptococcus gattii infections. The infection typically initially attacks the lungs followed by spreading to other organs as well as the central nervous system (CNS) [Zhu et al., 2014]. The CNS is usually infected by an opportunistic infection when the CD4 T-lymphocytes density is lower than 200 cells per μL. |
| Technique | Analytic |
| Analysis Method | Flow Cytometry Analysis |
| ELISA kits | ELISA kits (Shanghai Westang Bio-tech, Shanghai, China). |
| Assay Data | None |
| Validation Techniques used | ELISA Based, qRT-PCR, Flow Cytometry, immunohistochemistry techniques, immunofluorescence assay, western blotting |
| Up Regulation Down Regulation | Decrease |
| Sequence Data | None |
| External Link | None |
