| DB ID | MyCo_3283 |
| Title | Innate phase production of IFN-γ by memory and effector T cells expressing early activation marker CD69 during infection with Cryptococcus deneoformans in the lungs |
| Year | 2024 |
| PMID | 38700335 |
| Fungal Diseases involved | Cryptococcus deneoformans infection |
| Associated Medical Condition | None |
| Genus | Cryptococcus |
| Species | deneoformans |
| Organism | Cryptococcus deneoformans |
| Ethical Statement | None |
| Site of Infection | Lungs |
| Opportunistic invasive | None |
| Sample type | Biopsy |
| Sample source | Extracted Tissue |
| Host Group | Animal |
| Host Common name | Mice |
| Host Scientific name | Mus musculus |
| Biomarker Name | CD69 |
| Biomarker Full Name | CD69 |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | USA |
| Cohort | C57BL/6 mice, purchased from CLEA (Tokyo, Japan), were used as controls [wild-type (WT)]. CARD9 gene-disrupted [knockout (KO)], Dectin-2KO, and MincleKO mice were generated and established as described previously and backcrossed to C57BL/6 mice for more than eight generations. OT-II transgenic mice (OT-II mice), which express T cell receptor (TCR) α and β chains that recognize the major histocompatibility complex (MHC) class II Ib–restricted ovalbumin (OVA) peptide (residues 323–339) in a C57BL/6 background, were kindly provided by Dr. N. Ishii (Tohoku University, Sendai, Japan). |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | The two sister species, Cryptococcus neoformans (formerly C. neoformans var. grubii, serotype A) and C. deneoformans (formerly C. neoformans var. neoformans, serotype D), are yeast-type fungal pathogens characterized by thick capsules composed of polysaccharides such as glucuronoxylomannan and galactoxylomannan. These fungi grow in pigeon droppings and enter the lungs via an airborne route. While most healthy individuals experience asymptomatic infection, marked by granulomatous lesions in the lungs caused by these fungi, immunocompromised hosts with severely impaired cellular immunity, such as those with AIDS, frequently suffer from severe lung lesions and disseminated infections that extend to the central nervous system. |
| Technique | Analytic |
| Analysis Method | Flow Cytometry Analysis |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | Flow Cytometry |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
