| DB ID | MyCo_3276 |
| Title | Human MAIT cells are rapidly activated by Aspergillus spp. in an APC-dependent manner |
| Year | 2018 |
| PMID | 30059139 |
| Fungal Diseases involved | Invasive aspergillosis |
| Associated Medical Condition | None |
| Genus | Aspergillus |
| Species | terreus |
| Organism | Aspergillus terreus |
| Ethical Statement | Buffy Coats were obtained after written informed consent of blood donors (ethics committee approval 4357-03/15) |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Body fluid |
| Sample source | Blood |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | Granzyme |
| Biomarker Full Name | Granzyme |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | Germany |
| Cohort | Buffy Coats were obtained after written informed consent of blood donors (ethics committee approval 4357-03/15). PBMC were isolated by standard density gradient centrifugation with Biocoll (Biochrom AG). Subsequently, CD8+ (purity of 94% CD8+ cells, containing 81% CD3+ TC, 12% CD3+ CD56+ NKT cells and less than 3% CD3- CD56+ NK cells) and nonCD8+ cells were isolated by magnetic activated cell sorting using human CD8 MicroBeads (Miltenyi Biotec) or CD14+ monocytes (purity of 78% CD14+ cells) were isolated by using human CD14 MicroBeads (Miltenyi Biotec). MAIT cells were sorted from pre-enriched CD8+ cells for CD3+CD161+CD8+ with a purity of 90% (97% of this population were TCRVα7.2+). |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Fungal infections are a major threat for immunocompromised patients. Especially invasive aspergillosis is an important cause of morbidity and mortality in hematopoietic stem cell transplant and solid organ transplant recipients. The first line of defence by the innate immune system conducted by alveolar macrophages and neutrophilic granulocytes is followed by a robust T cell (TC) response triggered by dendritic cells traveling to lymph nodes and presenting pathogen-derived antigens to naïve TC. However, conventional TC require time for activation and expansion before they effectively combat pathogens. Several innate-like TC subtypes are known which may be active during this time lag. They include γδ TC, invariant natural killer TC and mucosal associated invariant T cells (MAIT). These cells are a subset of CD8+ unconventional TC expressing an evolutionary conserved invariant T cell receptor (TCR) recognizing antigens presented by the MHC class-I-related protein (MR1). They are located in various tissues like intestinal mucosa, lung and liver and comprise up to 10% of the TC population in the peripheral blood of human beings. Upon activation, they release pro-inflammatory cytokines, proliferate and are able to kill infected cells. |
| Technique | Analytic |
| Analysis Method | Flow Cytometry Analysis |
| ELISA kits | Human Perforin ELISAPRO kit (Mabtech AB) |
| Assay Data | None |
| Validation Techniques used | Flow Cytometry, Degranulation Assay, ELISA |
| Up Regulation Down Regulation | Decrease |
| Sequence Data | None |
| External Link | None |
