| DB ID | MyCo_3102 |
| Title | β-D-glucan and S-adenosylmethionine serum levels for the diagnosis of Pneumocystis pneumonia in HIV-negative patients: a prospective study |
| Year | 2011 |
| PMID | 20970450 |
| Fungal Diseases involved | Pneumocystis pneumonia |
| Associated Medical Condition | None |
| Genus | Pneumocystis |
| Species | jirovecii |
| Organism | Pneumocystis jirovecii |
| Ethical Statement | The study was approved by the institutional review board of the Leiden Uni- versity Medical Center and all patients provided written in- formed consent for participation in the study. |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Body fluid |
| Sample source | Serum |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | BDG |
| Biomarker Full Name | 1-3-beta-D-Glucan |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | Netherlands |
| Cohort | In this prospective observational study, consecutive HIV- negative, adult immunocompromised patients suspected of having PCP based on presentation and chest imaging were enrolled during admission in the Leiden University Medical Center, a tertiary care and teaching hospital in The Nether- lands.Videobronchoscopy was performed and a segment of an involved lung zone was lavaged using 20 ml aliquots. In total, 31 patients enrolled (21 PCP-positive, 10 PCP-negative). |
| Cohort No. | 31 |
| Age Group | 50-70 |
| P Value | None |
| Sensitivity | 0.009 |
| Specificity | 0.0089 |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Pneumocystis pneumonia (PCP), caused by Pneumocystis jirovecii is an important cause of morbidity and mortality in patients with human immunodeficiency virus (HIV) infec- tion and other conditions associated with immunosuppres- sion. The diagnosis of PCP is based on microscopy methods (silver, giemsa and immunoflorescent staining) and real-time PCR performed on broncho-alveolar lavage (BAL) samples obtained from patients with a compatible clinical picture. Microscopy techniques are limited by their sensitivity and time demanding procedures. Currently used real-time PCR methods to detect P. jirovecii yield high sen- sitivity but might lack the required specificity by detecting P. jirovecii also in patients who are colonized but do not suffer from PCP. Furthermore, the need for both sensi- tive and specific serum tests for PCP becomes particularly evident when invasive diagnostic procedures cannot be performed due to a patient’s clinical condition. Hence, a number of serum markers, including (1/3)-b-D-glucan (b-D-glucan) and S-adenosylmethionine (AdoMet) levels were recently studied for their ability to discriminate be- tween PCP and other pulmonary conditions. |
| Technique | ELISA |
| Analysis Method | FDA approved Fungitell assay |
| ELISA kits | None |
| Assay Data | FDA- Fungitell®, Cape Cod International, Inc.; Falmounth, MA, USA |
| Validation Techniques used | FDA approved Fungitell assay |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
