| DB ID | MyCo_2933 |
| Title | Rapid microbial identification and colistin resistance detection via MALDI-TOF MS using a novel on-target extraction of membrane lipids |
| Year | 2020 |
| PMID | 33299017 |
| Fungal Diseases involved | Fungal infection |
| Associated Medical Condition | None |
| Genus | None |
| Species | None |
| Organism | None |
| Ethical Statement | None |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Lipid |
| Sample source | Lipids |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | Lipid A |
| Biomarker Full Name | Lipid A |
| Biomarker Type | Diagnostic |
| Biomolecule | Lipid |
| Geographical Location | USA |
| Cohort | We applied the FLAT method to 149 replicates of 35 bacterial strains as follows: 20 unique clinical and laboratory adapted isolates of Gram-negative bacteria (E. coli, K. pneumoniae, A. baumannii, P. aeruginosa, M. morganii, and S. marcescens) including seven strains transformed with the mcr-1 plasmid, and 15 unique clinical and laboratory adapted isolates of Gram-positive bacteria (S. aureus, B. cereus, and B. mycoides). In addition, we applied the FLAT method to four replicates of two laboratory adapted isolates of Candida auris. We compared FLAT results to results with lipid microextraction on 157 replicates of the same and similar strains of the same bacterial and fungal species. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Rapid administration of appropriate antimicrobial therapy significantly improves patient survival, making rapid microbial diagnostics a critical need. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS or simply MALDI) of proteins in the 2000 to 20,000 m/z range is the clinical standard for microbial identification (ID). MALDI protein ID offers a simple workflow, low cost-per-sample, and rapid time to result using colonies from solid growth medium. Current MALDI ID systems include MALDI Biotyper (Bruker Daltonics, Billerica, MA) and VITEK MS (bioMérieux, France). To achieve the speed and limited hands-on time necessary for clinical use, we developed a new extraction method—FLAT (“fast lipid analysis technique”), that requires less than an hour of elapsed time and is highly parallelizable and amenable to automation. By directly extracting lipids on the heated surface of a stainless steel MALDI plate, FLAT minimizes handling steps and thus demands significantly less hands-on time than previous lipid analysis methods. Furthermore, FLAT can identify bacteria and fungi, while simultaneously detecting antimicrobial resistance signals that are not reported by current clinical MALDI ID systems, thus demonstrating the significant potential of FLAT in a clinical diagnostic laboratory setting. |
| Technique | Analytic |
| Analysis Method | Fast Lipid Analysis Technique or FLAT |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | Fast Lipid Analysis Technique or FLAT |
| Up Regulation Down Regulation | None |
| Sequence Data | None |
| External Link | None |
