| DB ID | MyCo_2737 |
| Title | Development of a Simple and Robust Whole Blood Assay with Dual Co-Stimulation to Quantify the Release of T-Cellular Signature Cytokines in Response to Aspergillus fumigatus Antigens |
| Year | 2021 |
| PMID | 34201183 |
| Fungal Diseases involved | Aspergillosis |
| Associated Medical Condition | None |
| Genus | Aspergillus |
| Species | fumigatus |
| Organism | Aspergillus fumigatus |
| Ethical Statement | The study was conducted according to the guidelines of the Declaration of Helsinki, and approved by the Ethics Committees of the University of Wuerzburg (protocol codes 34/15; 9/3/2015 and 178/16; 18/12/2018) and the National Health Service, Health Research Authority (REC reference 19/SC/0645; 07/01/2020). |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Body fluid |
| Sample source | Blood |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | IL-5 |
| Biomarker Full Name | Interleukin-5 |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | Wales |
| Cohort | For studies in patients with chronic lung diseases, 2 patients with cystic fibrosis (CF) and elevated Aspergillus-specific immunoglobulin E (age 41–47, 1 male, 1 female), 3 patients with allergic bronchopulmonary aspergillosis (ABPA, age 33–83, 1 male, 2 female), 4 patients with chronic pulmonary aspergillosis (CPA, age 60–80, 3 male, 1 female), and 5 control patients with non-mold-related interstitial lung diseases (age 56–90, 4 male, 1 female) were enrolled at the University Hospital of Wales. |
| Cohort No. | 9 patients + 5 Control |
| Age Group | 33-90 |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | -2 |
| Pathway | None |
| Disease Introduction Mechanism | Humans inhale hundreds of airborne conidia of the ubiquitous mold Aspergillus fumigates daily, resulting in constant activation of the innate and adaptive immune system in order to clear the fungus from the lungs and maintain protective tolerance. Imbalance of immunity can lead to a wide spectrum of A. fumigatus-associated diseases, ranging from invasive aspergillosis to hypersensitivity disorders. As fine-tuning of the anti-Aspergillus response by T-cellular cytokines has major implications for protective immunity, recovery from invasive infection, and inflammatory immunopathology, deeper understanding of cytokine signatures may lead to advances in the diagnosis and treatment of invasive mycoses and mold-associated hypersensitivity syndromes |
| Technique | ELISA |
| Analysis Method | ELISA Based |
| ELISA kits | ELISA- Max Deluxe Sets (Biolegend, San Diego, CA, USA) |
| Assay Data | None |
| Validation Techniques used | ELISA |
| Up Regulation Down Regulation | None |
| Sequence Data | None |
| External Link | None |
