| DB ID | MyCo_2731 |
| Title | Interferon-gamma and tumor necrosis factor-alpha determine resistance to Paracoccidioides brasiliensis infection in mice |
| Year | 2000 |
| PMID | 10793093 |
| Fungal Diseases involved | Paracoccidioidomycosis |
| Associated Medical Condition | None |
| Genus | Paracoccidioides |
| Species | brasiliensis |
| Organism | Paracoccidioides brasiliensis |
| Ethical Statement | None |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Biopsy |
| Sample source | Extracted cell supernatant |
| Host Group | Animal |
| Host Common name | Mice |
| Host Scientific name | Mus musculus |
| Biomarker Name | IFNγ |
| Biomarker Full Name | Interferon gamma |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | Germany |
| Cohort | Breeding pairs of mice with targeted disruption of the IFN- (GKO mice)15 and TNF- receptor p55 (p55KO) genes16 were obtained from Jackson Laboratories (Bar Harbor, ME) and Amgen Institute (Toronto, Canada), re-spectively. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Paracoccidioidomycosis (PCM), the major systemic mycosis in Latin America, is caused by the thermally dimorphic fungus Paracoccidioides brasiliensis (Pb). The infection is acquired by inhalation of airborne propagules produced by the fungal mycelium form, which then change into the pathogenic yeast form when at core body temperature. The yeast forms in the mammalian host can be eliminated by immune competent cells or disseminate to tissues by the lymphatic and hematogenous route. After infection there are no immediate signs or symptoms of the disease, characterizing silent PCM but the host develops a specific immune response against fungal antigens which can be detected by a skin test. In endemic areas, up to 60% of asymptomatic individuals may be skin test positive. |
| Technique | ELISA |
| Analysis Method | ELISA Based |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | Two-sandwich enzyme-linked immunosorbent assay |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
