| DB ID | MyCo_2678 |
| Title | Changes in Antioxidant Enzymes Activity and Metabolomic Profiles in the Guts of Honey Bee (Apis mellifera) Larvae Infected with Ascosphaera apis |
| Year | 2020 |
| PMID | 32640515 |
| Fungal Diseases involved | Chalkbrood disease |
| Associated Medical Condition | None |
| Genus | Ascosphaera |
| Species | apis |
| Organism | Ascosphaera apis |
| Ethical Statement | None |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Body fluid |
| Sample source | Honey bee larvae |
| Host Group | Insect |
| Host Common name | Honey bee |
| Host Scientific name | Apis mellifera |
| Biomarker Name | 5-oxo-ETE |
| Biomarker Full Name | 5-Oxo-eicosatetraenoic acid |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | china |
| Cohort | None |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | The fungus Ascosphaera apis, an obligate fungal pathogen of honey bee brood, causes chalkbrood disease in honey bee larvae. Larvae aged 3–4 days are most susceptible to A. apis infection through the ingestion of food containing fungal spores delivered by contaminated nurse bees. A. apis infection damages the gut lining of the host, and the fungal hyphae penetrate into the gut wall of the infected larva. Transcriptomic studies of A. apis indicated that fungal transcripts encoding chitinases may contribute to the penetration of the larval gut during host invasion by A. apis. A. apis infection not only acts as a direct disease stressor causing chalkbrood in honey bees, but also interacts with other biotic and abiotic stressors. Worker honey bees from chalkbrood-infected colonies exhibit significantly elevated deformed wing virus (DWV) viral load. Common honey bee viruses, such as DWV, could infect and replicate in A. apis. More severe symptoms can be found in A. apis-infected larvae, because the virulence of A. apis is likely to increase due to chilling stress. |
| Technique | ELISA |
| Analysis Method | ELISA Based |
| ELISA kits | sandwich ELISA kit (mlbio, Shanghai, China) |
| Assay Data | None |
| Validation Techniques used | ELISA, UHPLC-QTOF-MS |
| Up Regulation Down Regulation | Increase |
| Sequence Data | None |
| External Link | None |
