| DB ID | MyCo_2666 |
| Title | Development of a nano-gold immunodiagnostic assay for rapid on-site detection of invasive aspergillosis |
| Year | 2019 |
| PMID | 31355743 |
| Fungal Diseases involved | Invasive aspergillosis |
| Associated Medical Condition | None |
| Genus | Aspergillus |
| Species | spp. |
| Organism | Aspergillus spp. |
| Ethical Statement | The raising of antibodies in rabbits was approved by the IAEC committee, Agharkar Research Institute, Pune, India (IAEC/2013/02). The clinical part of the study was approved by the IEC committee of PGIMER, Chandigarh, India (P-296, vide letter no. PGI/IEC/2012/1340–41). |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Body fluid |
| Sample source | Serum |
| Host Group | Animal |
| Host Common name | Rabbit |
| Host Scientific name | Oryctolagus cuniculus |
| Biomarker Name | GM |
| Biomarker Full Name | Galactomannan |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | India |
| Cohort | The clinical isolates A. fumigatus NCCPF 770078 and A. flavus NCCPF 761090 were provided by the Post Graduate Institute of Medical Education and Research, Chandigarh, India. Cultures were grown and maintained by regular subculturing on yeast peptone glucose [(YPG; yeast extract 0.3 %, peptone 0.5 %, glucose 1 % (w/v)] agar medium at 37 °C. New Zealand White rabbits were immunized with purified A. fumigatus and A. flavus GM fractions mixed with complete Freund’s adjuvant. |
| Cohort No. | 109 Samples |
| Age Group | None |
| P Value | None |
| Sensitivity | 0.8 |
| Specificity | 0.932 |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Human invasive aspergillosis (IA) leads to high mortality, especially in immunocompromised patients. Over 10 million immunocompromised patients are at risk of IA and >200 000 cases are reported annually worldwide. Aspergillus fumigatus is the most common aetiological agent for aspergillosis globally, while Aspergillus flavus is more prevalent in India. |
| Technique | Immunological assay |
| Analysis Method | ELISA Based |
| ELISA kits | Platelia Aspergillus ELISA Kit (Bio-Rad Laboratories, Marne-La-Coquette, France) |
| Assay Data | None |
| Validation Techniques used | ELISA |
| Up Regulation Down Regulation | Increase |
| Sequence Data | None |
| External Link | None |
