| DB ID | MyCo_2638 |
| Title | Human Invariant Natural Killer T cells possess immune-modulating functions during Aspergillus infection |
| Year | 2015 |
| PMID | 26483428 |
| Fungal Diseases involved | Aspergillus infection |
| Associated Medical Condition | None |
| Genus | Aspergillus |
| Species | fumigatus |
| Organism | Aspergillus fumigatus |
| Ethical Statement | Research with blood samples from healthy volunteer donors was approved by the Ethics Committee of the University of Wuerzburg. Participants provided their written consent to participate in this study, and the Wuerzburg Ethics Committee approved this consent procedure. |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Body fluid |
| Sample source | Blood |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | IL-12 |
| Biomarker Full Name | Interleukin-12 |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | Germany |
| Cohort | Mononuclear cells from peripheral blood (PBMCs) were obtained from buffy coats of healthy volunteer donors by Ficoll (Biochrom GmbH) density gradient centrifugation. |
| Cohort No. | None |
| Age Group | None |
| P Value | p<.005 |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Natural killer T (NKT) cells compose a small subset of T lymphocytes that are restricted to recognition of glycolipids presented by the MHC class I-like molecule CD1d and express a T cell receptor (TCR) as well as NK cell markers (CD161/CD56). The human, predominantly studied type I NKT cells, also called invariant natural killer T (iNKT) cells, express an invariant TCR consisting of a Vα24-Jα18 α-chain paired with a Vβ11 β-chain. The function of murine iNKT cells in infection with A. fumigatus has been first described by Cohen et al., who showed that CD1d-dependent iNKT cell activation played a role in early pulmonary clearance of A. fumigatus. This iNKT cell activation was postulated to be induced by the recognition of fungal ß1,3-Glucans through Dectin-1 on DCs and subsequent IL-12 secretion which then stimulated iNKT cells. Albacker et al., described a glycolipid extracted from A. fumigatus that directly activated Inkt cells via a CD1d dependent, Dectin-1 independent mechanism. This glycosphingolipid, asperamide B, was the first fungal antigen shown to be presented by CD1d and to directly stimulate iNKT cells. However, there is very limited data about the interaction of human iNKT cells with A. fumigatus.Moreover, it is unclear whether all different CD1d expressing APCs interact equally with iNKT cells. |
| Technique | ELISA |
| Analysis Method | ELISA Based |
| ELISA kits | Multiplex ELISA Kit (Bio-Plex Pro Human Cytokine 27-plex Assay, Bio-Rad) |
| Assay Data | None |
| Validation Techniques used | ELISA, Flowcytometry |
| Up Regulation Down Regulation | Decrease |
| Sequence Data | None |
| External Link | None |
