| DB ID | MyCo_2612 |
| Title | Immunoassays based on Penicillium marneffei Mp1p derived from Pichia pastoris expression system for diagnosis of penicilliosis |
| Year | 2011 |
| PMID | 22205971 |
| Fungal Diseases involved | Penicilliosis |
| Associated Medical Condition | None |
| Genus | Penicillium |
| Species | marneffei |
| Organism | Penicillium marneffei |
| Ethical Statement | The use of patient serum samples was approved by Institutional Review Board of the University of Hong Kong/Hospital Authority Hong Kong West Cluster under protocol no. UW 04-278 T/600 for all samples collected since September 2004. Control sera were collected specifically for this study. Informed written consent was obtained from each subject. The use of control sera obtained from blood donors and patient sera from an already-existing collection was approved by the Ethics Committee of the Zhujiang Hospital of Southern Medical University, Guangzhou, China, with the following reference number: ZJYY-2010-YXJYZX-001. |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Body fluid |
| Sample source | Serum |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | Mp1p |
| Biomarker Full Name | Mannoprotein Mp1p |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | China |
| Cohort | Twenty serum specimens were obtained from 14 penicilliosis patients diagnosed by positive culture from blood or bone marrow aspirate specimens. |
| Cohort No. | 14 |
| Age Group | None |
| P Value | None |
| Sensitivity | 0.55 |
| Specificity | 0.996 |
| Positive Predictive Value | 0.846 |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Penicillium marneffei is an emerging fungus endemic in Southeast Asian regions, including Burma, Cambodia, Indonesia, Malaysia, Thailand, Vietnam, and southern parts of China. Penicilliosis, the systemic disease caused by P. marneffei, occurs in both immunocompetent and immunocompromised patients, particularly in AIDS patients. The infection mainly affects HIV-infected patients and is considered to be one of the most common opportunistic infections among AIDS patients in the endemic regions, and is therefore regarded as an indicator disease for AIDS. In addition, infections by P. marneffei have been reported in visitors who had travelled to the endemic regions. Penicilliosis is a disseminated and progressive disease with high mortality. However, successful therapeutic management has been hampered by an absence of rapid and accurate diagnosis due to its non-specific symptoms and similar biological characteristics to other common pathogenic fungi, such as Histoplasma capsulatum, Cryptococcus neoformans and various Aspergillus species. A number of diagnostic methods based on antibody as well as antigen detection have been developed, but they have not been used for routine diagnosis. |
| Technique | ELISA |
| Analysis Method | ELISA Based |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | ELISA |
| Up Regulation Down Regulation | Increase |
| Sequence Data | None |
| External Link | None |
