| DB ID | MyCo_2389 |
| Title | Identification of early biomarkers in a rabbit model of primary Candida pneumonia |
| Year | 2019 |
| PMID | 31387541 |
| Fungal Diseases involved | Candida pneumonia |
| Associated Medical Condition | None |
| Genus | Candida |
| Species | albicans |
| Organism | Candida albicans |
| Ethical Statement | All procedures involving animals were performed in accordance with the ethical standards of the participating institution and the Guidelines for the Humane Treatment of Laboratory Animals (Ministry of Science and Technology of the People’s Republic of China, Policy No. 2006 398), and were approved by the Institutional Animal Care and Use Committee of Shanghai Fifth People’s Hospital. |
| Site of Infection | Upper respiratory tract |
| Opportunistic invasive | Opportunistic |
| Sample type | Body fluid |
| Sample source | Blood |
| Host Group | Animal |
| Host Common name | Rabbit |
| Host Scientific name | Oryctolagus cuniculus |
| Biomarker Name | sCD163 |
| Biomarker Full Name | Soluble hemoglobin-haptoglobin scavenger receptor (sCD163) |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | China |
| Cohort | A total of 40, pathogen free, New Zealand white rabbits (males, 2 months old, 2.0–2.5 kg) were purchased from the Animal Center of the Shanghai Jiaotong University. The animals were reared in well ventilated, stainless steel, 60 × 80 cm rabbit cages (2 rabbits per cage) placed in a temperature-controlled room (20m2) at 20– 30 °C and 40–70% humidity. Lighting included a combination of natural light and fluorescence. Tap water and mixed pellet feed were provided daily. Rabbit cages and drinking bottles were sterilized using 0.1% benzalkonium bromide and bedding was changed every 2 days. The animals were observed for 5 days and used for experimentation in the absence of anomalous behavior. |
| Cohort No. | 40 Population |
| Age Group | None |
| P Value | p< 0.01 |
| Sensitivity | 0.8 |
| Specificity | 0.75 |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Candida albicans is an opportunistic pathogen, but since it also belongs to the normal fungal flora, positive sputum culture as the solely basis for the diagnosis of invasive Candida albicans pneumonia can easily lead to excessive antifungal therapy. Nevertheless, diagnosis of Candida pneumonia is a challenge, since Candida spp. are part of the normal flora and asymptomatic colonizers of various mucosal surfaces including the upper respiratory tract. There is a need to differentiate between primary isolated Candida lung invasion causing pneumonia and secondary hematogenously disseminated Candida reaching the lungs from elsewhere. Pneumonia caused by fungi is provisionally diagnosed by a combination of clinical and radiological findings, together with cultures and serology. |
| Technique | ELISA |
| Analysis Method | ELISA Based |
| ELISA kits | ELISA Kit (Guangzhou Jianlun Biological Technology Co., Ltd.). sTREM-1 (96 T kit, JL003211), PCT (JL002421), IL-6 (JL002052), IL-8 (JL001248), IL-10 (JL001132) and TNF-α (JL004161). |
| Assay Data | None |
| Validation Techniques used | ELISA |
| Up Regulation Down Regulation | Increase |
| Sequence Data | None |
| External Link | None |
