| DB ID | MyCo_2102 |
| Title | Phenotypic and functional evaluations of peripheral blood monocytes from chronic-form paracoccidioidomycosis patients before and after treatment |
| Year | 2014 |
| PMID | 25314914 |
| Fungal Diseases involved | Paracoccidioidomycosis |
| Associated Medical Condition | None |
| Genus | Paracoccidioides |
| Species | brasiliensis |
| Organism | Paracoccidioides brasiliensis |
| Ethical Statement | This study was approved by the Research Ethics Committee of FMB-UNESP (#3145/2009). Written informed consent to participate and to publish the data was obtained from all participants. In this study IRB was signed by all the adult patients. We had no IRB signed by the closest relative or the legal representative. |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Body fluid |
| Sample source | Blood |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | IL-1β |
| Biomarker Full Name | Interleukin-1β |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | Brazil |
| Cohort | Twenty-three CF PCM patients (PCM-p) from the Trop-ical Diseases Ward and Outpatient Clinic for Paracocci-dioidomycosis at the University Hospital, Faculdade de Medicina de Botucatu (FMB), UNESP–Univ. Estadual Paulista, Botucatu, SP, Brazil, were studied. |
| Cohort No. | 23 |
| Age Group | 37-60 |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Paracoccidioidomycosis (PCM) is a systemic and granu- lomatous mycosis caused by thermally dimorphic fungi of the Paracoccidioides brasiliensis complex and P. lutzii and is characterized by antigen-dependent im- munosuppression. The active disease presents two main clinical forms: the acute/subacute form (AF) and the chronic form (CF). PCM patients (PCM-p) with the AF are typically young and normally show a short duration of the symptomatology (median of two months), exhibiting clinical manifestations that involve organs rich in the mononuclear phagocytic system (e.g. bone marrow, liver, spleen). CF usually affects adult males who present a long duration of the symptomatology (>6 months) and exhibit predominant pulmonary and mucocutaneous involvement. After treatment, numerous CF patients present seque-lae, especially pulmonary fibrosis and emphysema, and Addison’s syndrome. |
| Technique | ELISA |
| Analysis Method | ELISA Based |
| ELISA kits | Cytokine Duo-Set Kit (R&D Systems, Minneapolis, USA), flow cytometry and the Human Inflammation Kit, BD™, Cyto- metric Bead Array (BD). |
| Assay Data | None |
| Validation Techniques used | ELISA, Flow Cytometry Analysis |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
