| DB ID | MyCo_2054 |
| Title | The evaluation of local oxidative/nitrosative stress in patients with pityriasis versicolor: a preliminary study |
| Year | 2016 |
| PMID | 27329971 |
| Fungal Diseases involved | Pityriasis versicolor |
| Associated Medical Condition | None |
| Genus | Malassezia |
| Species | furfur |
| Organism | Malassezia furfur |
| Ethical Statement | The study was approved by the local ethical committee of Sutcu Imam University, Medical Faculty, Kahramanmaras, Turkey. |
| Site of Infection | Skin |
| Opportunistic invasive | None |
| Sample type | Biopsy |
| Sample source | Keratinous tissue |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | MDA |
| Biomarker Full Name | Malondialdehyde |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | Turkey |
| Cohort | Participants were men and women older than 18 years of age with a clinical diagnosis of PV and positive microscopy on a potassium hydroxide (KOH) preparation. Subjects were selected randomly. None of the patients showed signs of either mental or physical symptoms of zinc deficiency. None of them had alcohol abuse problems or smoked. No female patients were pregnant. People performing regular sports and hard works were not included in the study. Patients with liver, renal and cardiac diseases and also those on immunosuppressive drugs or known to have HIV infection were also excluded from the study. The distribution of lesions of PV by regions of the body was classified into three groups: (i) Mild: involvement of only one region of the body (neck, anterior and posterior thorax); (ii) Moderate: involvement of more than one region of the body and less than or equal to three regions of the body (anterior thorax + posterior thorax + abdominal region; anterior thorax + posterior thorax + dorsal region; anterior thorax + upper limbs; anterior thorax + posterior thorax + neck; forearms, abdominal region, dorsal region); (iii) Disseminated: involvement of more than three regions of the body (neck + trunk + upper limbs + lower limbs + armpits + groin region). Samples were obtained from Wood’s lamp and KOH examinations positive areas in the skin with a sterile scalpel by scraping from patients with PV. The skin scraping samples in controls were taken until the bleeding point from normal apparently skin and they had negative Wood lamp examination. The areas of control skin scraping were similar to the areas of patients skin scraping. A portion of scraping samples of both groups was placed in sterile Petri dishes for microbiologic analysis. The other portion of both groups was stored at -80 °C until analysis for biochemical analysis. |
| Cohort No. | None |
| Age Group | None |
| P Value | p<0.001 |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Pityriasis versicolor (PV) is a disease with worldwide distribution. It is a superficial infection of stratum corneum caused by Malassezia spp. It is characterised by scaly hypopigmented or hyperpigmented lesions with minimal pruritus. The genus Malassezia includes a group of lipophilic yeast whose natural habitat is the human skin. As other microorganisms of the normal human cutaneous commensal flora, Malassezia produces an enzyme with lipase activity, as demonstrated by its ability to release fatty acids from the triglycerides of the sebum. Also, Malassezia spp is dimorphic, existing in both yeast and mycelial phases. PV is caused by conversion from the yeast to the mycelial form, which is then able to invade stratum corneum, penetrating both between and through the corneocytes. The cause or causes of the change of the yeast from saprophytic or yeast phase to parasitic or mycelial phase remains unclear. As histopathological image of PV, samples which are taken from PV lesions show that the Malassezia yeast can be present in all layers of stratum corneum. Otherwise, healthy individuals with PV produce fewer lymphokines compared with normal controls. In addition, a large number of Langerhans cells are found in the epidermis in PV lesions. Neutrophils and monocytes appear to be important in the defence against Malassezia. |
| Technique | ELISA |
| Analysis Method | ELISA Based |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | ELISA |
| Up Regulation Down Regulation | Increase |
| Sequence Data | None |
| External Link | None |
