| DB ID | MyCo_1902 |
| Title | Differential dendritic cell responses to cell wall mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis |
| Year | 2018 |
| PMID | 30429436 |
| Fungal Diseases involved | Oral candidiasis |
| Associated Medical Condition | None |
| Genus | Candida |
| Species | albicans |
| Organism | Candida albicans |
| Ethical Statement | All procedures were approved by the Institutional Animal Use and Care Committee of Chulalongkorn University Lab Animal Center (Protocol number 1573005). |
| Site of Infection | Mouth |
| Opportunistic invasive | Opportunistic |
| Sample type | Biopsy |
| Sample source | Bone marrow-derived DCs |
| Host Group | Animal |
| Host Common name | Mice |
| Host Scientific name | Mus musculus |
| Biomarker Name | Mannan |
| Biomarker Full Name | Mannosylated glycoproteins |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | Thailand |
| Cohort | Three Candida species were selected for our study: C. albicans (ATCC 24433), C. parapsilosis (ATCC 90018), and C. dubliniensis (NCPF 3949 or MYA-646). The strains chosen for C. albicans and C. parapsilosis are reference strains for quality control and antifungal drug susceptibility testing, whereas the strain for C. dubliniensis is the only available standard strain (Microbiologics Inc., St. Cloud, MN, USA). The fungi were grown on YPD agar (2% yeast extract, 1% peptone, 2% dextrose, and 2% agar) (HiMedia, Mumbai, India and Ajax Finechem, NSW, Australia) at 30°C for 2 days. The colonies were subcultured in YPD broth at 30°C with 200 rpm shaking for 8 h to reach the log phase. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Oral candidiasis is a common problem in the elderly and in immunocompromised individuals, whose defense mechanisms are impaired locally or systemically. Local and systemic predisposing factors leading to a pathogenic condition include ill-fitting prosthodontic appliances, xerostomia, malnutrition, and hormonal and endocrine disorders. Although the most common species responsible for oral candidiasis is C. albicans, a shift toward non-albicans Candida species (NACs) has been reported in recent decades because of the widespread use of broad-spectrum antibiotics and prolonged use of antibiotics and antifungal therapy. |
| Technique | ELISA |
| Analysis Method | ELISA Based |
| ELISA kits | ELISA Kit (Biolegend and eBioscience, San Diego, CA, USA) |
| Assay Data | None |
| Validation Techniques used | ELISA |
| Up Regulation Down Regulation | Increase |
| Sequence Data | None |
| External Link | None |
