| DB ID | MyCo_1839 |
| Title | Development of surrogate markers for oral immunisation against Candida albicans |
| Year | 2003 |
| PMID | 12531338 |
| Fungal Diseases involved | Oral candidiasis |
| Associated Medical Condition | None |
| Genus | Candida |
| Species | albicans |
| Organism | Candida albicans |
| Ethical Statement | None |
| Site of Infection | Mouth |
| Opportunistic invasive | Opportunistic |
| Sample type | Body fluid |
| Sample source | Saliva |
| Host Group | Animal |
| Host Common name | Mice |
| Host Scientific name | Mus musculus |
| Biomarker Name | IL-4 |
| Biomarker Full Name | Interleukin-4 |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | Australia |
| Cohort | Male BALB/c (H-2d) and DBA/2 mice (H-2d), 6–8 weeks old. Candida albicans isolate 3630 was obtained from the National Reference Laboratory, Royal North Shore Hospital, Sydney, Australia and blastospores collected from Sabouraud dextrose broth by centrifugation, washed and adjusted to 108 blastospores/ml in PBS until use. |
| Cohort No. | None |
| Age Group | None |
| P Value | p<0.05 |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Progress in development of mucosal vaccines has been restricted through a lack of validated surrogate markers of protection to aid in optimising formulation, dose and vaccination strategy. Mucosal candidiasis manifest as stomatitis or vaginitis, is a common and distressing problem, while more extensive upper gastrointestinal candidiasis in immune suppressed subjects predisposes to life threatening invasive disease. Cellular immunity contains mucosal disease, while antibody enhanced phagocytic mechanisms are critical to the outcome of systemic infection. Thus a vaccine to prevent or contain mucosal infection with Candida albicans would be of considerable clinical benefit. |
| Technique | ELISA |
| Analysis Method | ELISA Based |
| ELISA kits | ELISA Kit (Pharmingen, San Diego, CA) |
| Assay Data | None |
| Validation Techniques used | ELISA, RT-PCR |
| Up Regulation Down Regulation | Increase |
| Sequence Data | None |
| External Link | None |
