| DB ID | MyCo_1623 |
| Title | H-ficolin binds Aspergillus fumigatus leading to activation of the lectin complement pathway and modulation of lung epithelial immune responses |
| Year | 2015 |
| PMID | 26133042 |
| Fungal Diseases involved | Aspergillus fumigatus infection |
| Associated Medical Condition | None |
| Genus | Aspergillus |
| Species | fumigatus |
| Organism | Aspergillus fumigatus |
| Ethical Statement | Sampling of BAL from lung transplant patients from the Royal Brompton and Harefield NHS Foundation Trust was performed under Biomedical Research Unit ethics approval (RBH/AS1). |
| Site of Infection | None |
| Opportunistic invasive | Invasive/Opportunistic |
| Sample type | Body fluid |
| Sample source | Bronchoalveolar lavage fluid (BALF) |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | H-ficolin |
| Biomarker Full Name | H-ficolin |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | UK |
| Cohort | All experiments were conducted using the A549 adenocar- cinomic human alveolar basal epithelial cell line as a model for type II alveolar epithelial cells. |
| Cohort No. | None |
| Age Group | None |
| P Value | p<0 0001 |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Aspergillus fumigatus is the commonest mould pathogen in the developed world and is the primary causative species of the potentially fatal, invasive aspergillosis (IA) in immuno- compromised patients. IA is an increasing problem because of the emergence of a number of antifungal-re-sistant strains and even if treated effectively, IA harbours an infection-associated mortality rate between 30 and 80%. Transplant patients are at particular risk and it is estimated that up to one in four lung or heart transplant patients will succumb to this infection. IA propagated through the inhalation of small hydrophobic spores (conidia), which penetrate deep into the alveolar space where they germinate, invading the vasculature and disseminating to other organs such as the brain and skin. |
| Technique | ELISA |
| Analysis Method | ELISA Based |
| ELISA kits | ficolin-3 human ELISA kit (Hycult Biotech, Uden, the Netherlands). |
| Assay Data | None |
| Validation Techniques used | ELISA |
| Up Regulation Down Regulation | Increase |
| Sequence Data | None |
| External Link | None |
