| DB ID | MyCo_1611 |
| Title | Inhibition of Galectin-3 Impairs Antifungal Immune Response in Fungal Keratitis |
| Year | 2022 |
| PMID | 35437453 |
| Fungal Diseases involved | Fungal keratitis |
| Associated Medical Condition | None |
| Genus | Aspergillus |
| Species | fumigatus |
| Organism | Aspergillus fumigatus |
| Ethical Statement | This study was approved by the Zhongshan Ophthalmic Center Medical Science Research Ethics Committee (protocol number: 2020KYPJ115). |
| Site of Infection | Eye |
| Opportunistic invasive | Opportunistic |
| Sample type | Macromolecule |
| Sample source | Extracted RNA |
| Host Group | Animal |
| Host Common name | Mice |
| Host Scientific name | Mus musculus |
| Biomarker Name | Galectin-3 |
| Biomarker Full Name | Galectin-3 |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | China |
| Cohort | The tissue samples, ocular image, periodic acid-Schiff Stain (PAS), and microbial culture esults were collected from patients who were clinically diagnosed with fungal keratitis by corneal scraping culture and received corneal transplantation from May 2020 to May 2021 at the Zhongshan Ophthalmic Center. 150 female C57BL/6N mice (6-8 weeks old), weighing 18-21 g, were purchased from Beijing Vital River Laboratory Animal Technology Co. Ltd., and the construction of galectin-3 complete knockout mice was commissioned by Saiye Biology (Strain name: C57BL/6NLgals3em1cyagen, strain number: KOCMP-16854-Lgals3- B6N-VA). The mice were divided into four groups: wild type group, wild type fungal keratitis group, galectin-3-/- group, and galectin-3-/- fungal keratitis group. Animal breeding and experiments were carried out in the Specific Pathogen- Free (SPF) animal room of the Experimental Animal Center of Sun Yat-sen University Zhongshan Ophthalmic Center. All animals were treated following the guidelines provided by the Ophthalmology and Vision Research Animal Use Vision and Ophthalmology Research Association and were approved by the Zhongshan Ophthalmic Center Institutional Review Committee (ethics number: 2020-011). All laboratory animal use followed the Association for Research in Vision and Ophthalmology (ARVO) requirements. |
| Cohort No. | 150 female mice |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Fungal keratitis (FK), one of the most detrimental ocular diseases caused by a fungal infection, loomed so large to be a key health issue of great importance. There are at least 100,000 known species of fungi. However, fewer than 500 have been proved to cause disease in animals, including human beings. The incidence rate of aspergillus is the highest among the pathogenic fungi, according to epidemiologic studies. The immunological mechanism of microbial infectious disease includes both pathogen clearance and anti-infection immune response. The microbial ocular disease has been of great interest to us for over a decade |
| Technique | ELISA |
| Analysis Method | ELISA Based |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | ELISA, qRT-PCR, Flow Cytometry, Immunofluorescence Staining, Two-Photon Microscopy (TPM) |
| Up Regulation Down Regulation | None |
| Sequence Data | None |
| External Link | None |
