| DB ID | MyCo_1604 |
| Title | Detection of urinary excreted fungal galactomannan-like antigens for diagnosis of invasive aspergillosis |
| Year | 2012 |
| PMID | 22900046 |
| Fungal Diseases involved | Invasive aspergillosis |
| Associated Medical Condition | None |
| Genus | Aspergillus |
| Species | fumigatus |
| Organism | Aspergillus fumigatus |
| Ethical Statement | Mouse experiments were conducted at the Albert Einstein College of Medicine, Bronx, NY, following a protocol approved by the Institutional Animal Care and Use Committee (IACUC) of that institution (Protocol number: 20090404); imaging procedures were performed under isofluorane anesthesia according to protocol. Experiments with the Guinea Pig model of invasive aspergillosis were conducted at the San Antonio Center for Medical Mycology of the University of Texas Health Science Center, following protocols approved by IACUC (Protocol number: 00101G). All studies involving human urine samples were approved by the Johns Hopkins Medicine Institutional Review Board (IRB) (collection of urine from consented human volunteers, study number: NA_00029178; use of previously collected, de-identified, banked urine samples, study number: NA_00027228); written consent forms are in file. |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Body fluid |
| Sample source | Urine |
| Host Group | Animal |
| Host Common name | Hartley Guinea Pigs |
| Host Scientific name | Cavia porcellus |
| Biomarker Name | GM-reactive MAb476 |
| Biomarker Full Name | Galactomannan-reactive Monoclonal Antibody 476 |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | USA |
| Cohort | Urine samples were collected from immunosuppressed Hartley Guinea Pigs (GP), infected via an inhalational challenge with aerosolized Af293 conidia at days 0, 3, 5 or 7 post-infection. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Mortality associated with invasive aspergillosis (IA) is high despite availability of potent antifungal drugs. Delayed diagnosis partly contributes to this poor outcome, since classical microbiological methods are insensitive. Culture-independent detection of several fungal components have shown promise for earlier diagnosis. |
| Technique | ELISA |
| Analysis Method | ELISA Based |
| ELISA kits | ‘GM-EIA’ Platelia Aspergillus EIA, BioRad Inc., Hercules, CA. |
| Assay Data | None |
| Validation Techniques used | ELISA, LFD |
| Up Regulation Down Regulation | None |
| Sequence Data | None |
| External Link | None |
