| DB ID | MyCo_1390 |
| Title | E-NTPDase and E-ADA activities in rats experimental infected by Cryptococcus neoformans |
| Year | 2014 |
| PMID | 25278365 |
| Fungal Diseases involved | Cryptococcosis |
| Associated Medical Condition | None |
| Genus | Cryptococcus |
| Species | neoformans |
| Organism | Cryptococcus neoformans |
| Ethical Statement | This study was approved by the Ethics and Animal Welfare Committee of the Federal University of Santa Maria (UFSM) under protocol number 073/2012. |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Body fluid |
| Sample source | Serum |
| Host Group | Animal |
| Host Common name | Rat |
| Host Scientific name | Rattus norvegicus |
| Biomarker Name | E-NTPDase |
| Biomarker Full Name | E-NTPDase |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | Brazil |
| Cohort | A total of 35 adult rats, males, with a mean age of 90 days, and weighing 298 ( 29) grams were used in this study. They were kept in cages with 6 animals each in experimental facilities under controlled temperature and humidity (25 8C; 70%), fed with commercial feed, with water ad libitum, and submitted to a period of 7 days for adaptation. |
| Cohort No. | 35 |
| Age Group | None |
| P Value | p<0.01 |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Cryptococcosis is an invasive fungal infection caused inhalation of Cryptococcus neoformans or Cryptococcus gattii. C. neoformans is an encapsulated yeast that causes life-threatening illness predominantly in immunocompromised hosts. C. neoformans var. grubii is the most common variety responsible for more than 80% of all cryptococcal infections and also for more than 99% of the infections in AIDS patients. Previous studies show that intratracheal inocula-tion of rats with C. neoformans produces similarities to the course of pulmonary infection in immunocompetent humans. The infection may be disseminated via blood or lymph from the lungs to other organs, especially to the central nervous system (CNS), if the primary infection is mild or asymptomatic. The mechanism of host defense against C. neoformans infection in the CNS and lungs apparently occurs via Th1 cell-mediated effectors. However, the response mediated by antibodies to C. neoformans seems to depend on the association between Th1 and Th2 cytokines. |
| Technique | Analytic |
| Analysis Method | Colorimetric assay |
| ELISA kits | ELISA using commercial kits (eBIOSCIENCE1, San Diego, USA) |
| Assay Data | None |
| Validation Techniques used | ELISA, Colorimetric assay, Spectrophotometric Analysis |
| Up Regulation Down Regulation | Increase |
| Sequence Data | None |
| External Link | None |
