| DB ID | MyCo_1349 |
| Title | Oxidative stress in rats experimentally infected by Sporothrix schenckii |
| Year | 2017 |
| PMID | 28286151 |
| Fungal Diseases involved | Sporotrichosis |
| Associated Medical Condition | None |
| Genus | Sporothrix |
| Species | schenckii |
| Organism | Sporothrix schenckii |
| Ethical Statement | The procedure was approved by the Animal Welfare Committee of Federal University of Santa Maria (UFSM). |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Body fluid |
| Sample source | Blood |
| Host Group | Animal |
| Host Common name | Rat |
| Host Scientific name | Rattus norvegicus |
| Biomarker Name | CAT |
| Biomarker Full Name | Catalase |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | Brazil |
| Cohort | Immunocompetent adult male rats (n¼ 39; Rattus norvegicus) with an average of 60 days-old and 246 ± 45 g of weight were used. Thirty rats were divided into two groups: the group A (uninfected, n= 18) and the group B (infected by S. schenckii, n=21). Blood samples were collected on days 15, 30 and 40 post-infection (PI). |
| Cohort No. | 30 |
| Age Group | None |
| P Value | p<0.01 |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Sporothrix schenckii is the etiologic agent of a disease called sporotrichosis with worldwide distribution, and great impact on public health. This disease affects the skin and lymphatic vessels near the infected tissue and it may spread to bones, joints, and muscles of humans and other mammals, such as cats, dogs, rats, and horses. Skin lesions might be supercial with nodular ulcers, compromising lymphatic vessels. The systemic form of the disease is characterized by visceral lesions, which leads to oxidative stress due to an imbalance in the oxidant and antioxidant status, causing further harm to the patient. |
| Technique | Assay |
| Analysis Method | Catalase Assay |
| ELISA kits | ELISA Kit (eBIO-SCIENCE®) |
| Assay Data | None |
| Validation Techniques used | Catalase Assay, Superoxide dismutase Assay, Lipid peroxidation Assay, ELISA |
| Up Regulation Down Regulation | Increase |
| Sequence Data | None |
| External Link | None |
