| DB ID | MyCo_1055 |
| Title | Clinical Performance of Aspergillus PCR for Testing Serum and Plasma: a Study by the European Aspergillus PCR Initiative |
| Year | 2015 |
| PMID | 26085618 |
| Fungal Diseases involved | Aspergillosis |
| Associated Medical Condition | None |
| Genus | Aspergillus |
| Species | spp. |
| Organism | Aspergillus spp. |
| Ethical Statement | The study was approved by local ethical and research and development boards. |
| Site of Infection | None |
| Opportunistic invasive | Invasive |
| Sample type | Body fluid |
| Sample source | Serum |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | ITS |
| Biomarker Full Name | Internal Transcribed Spacer |
| Biomarker Type | Diagnostic |
| Biomolecule | Gene |
| Geographical Location | Italy |
| Cohort | The clinical evaluation was performed over 12 months by two centers experienced in Aspergillus PCR testing based in Public Health Wales, Microbiology Cardiff (PHW), Car-diff, United Kingdom, and the University of Würzburg (UKW), Würz- burg, Germany. The associ-ated analytical study confirmed that DNA concentrations were greater in plasma than those in serum. The aim of the current investigation was to confirm analytical findings by comparing the performance of Aspergillus PCR testing of plasma and serum in the clinical setting. Standardized Aspergillus PCR was performed on plasma and serum samples concurrently obtained from hematology patients in a multicenter retrospective anonymous case-control study, with cases diagnosed according to European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) consensus definitions (19 proven/probable cases and 42 controls). |
| Cohort No. | 19 Patients and 42 control |
| Age Group | 21-78 |
| P Value | None |
| Sensitivity | 0.684 |
| Specificity | 0.762 |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | None |
| Technique | PCR |
| Analysis Method | Aspergillus PCR |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | Aspergillus PCR |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
