OCA Atlas for 3NUC
3NUC Hydrolase date Dec 06, 1997
title Staphlococcal Nuclease, 1-N-Propane Thiol Disulfide To V23c Variant
authors R.Wynn, P.C.Harkins, F.M.Richards, R.O.Fox
compound source
Molecule: Staphylococcal Nuclease
Chain: Null
Ec: 3.1.31.1
Engineered: Yes
Mutation: V23c
Biological_unit: Monomer
 Organism_scientific: Staphylococcus Aureus
Expression_system: Escherichia Coli
Expression_system_strain: Ar120
Expression_system_vector: Pas1
symmetry Space Group: P 41
R_factor 0.171
crystal
cell
length a length b length c angle alpha angle beta angle gamma
48.300 48.300 63.100 90.00 90.00 90.00
method X-Ray Diffractionresolution 1.90 Å
ligand PR3, THP, CAenzyme Micrococcal nuclease. Micrococcal endonuclease. Hydrolase E.C.3.1.31.1
related structures by homologous chain: 1A2T
similarity Belongs to the thermonuclease family.[SNase]
catalytic activ. Endonucleolytic cleavage to 3'- phosphomononucleotide and 3'-phosphooligonucleotide end-products.
subcellular loc. Secreted (nuclease a) or membrane-bound (nuclease b).
gene NUC (S. aureus)
function Enzyme that catalyzes the hydrolysis of both dna and rna at the 5'position of the phosphodiester bond.
Data retrieval
  • Asymmetric unit, PDB entry: [header only] [complete with coordinates] (40 Kb) [Save to disk]
  • LPC: Ligand-Protein Contacts for 3NUC
  • CSU: Contacts of Structural Units for 3NUC
  • Likely Quarternary Molecular Structure file(s) for 3NUC
  • Retrieve 3NUC in mmCIF format [Save to disk]
    • View 3NUC in 3D
  • Protein Explorer, Easier to use and more powerful than RasMol. (Win and Mac only)
  • FirstGlance in Chemscape Chime (Install Chime freeware) This is good for easiest viewing of basic structure.
  • RasMol (Install RasMol freeware) Here's help on how to use RasMol.
    • Visual 3D analysis of 3NUC
  • STING, shows the sequence and maps the locations of sequence residues or residue ranges onto the 3D structure.
  • Noncovalent Bond Finder displays the closest contacts to ligand or interface, reports distances, walks over water bridges.
  • GRASS (Graphical Representation and Analysis of Structure Server)
    • Structure-derived information
  • Dipole moment, from Dipole Server at Weizmann Institute
  • Genome occurence of 3NUC's fold from GeneCensus
  • Classification of representative domains in scop (Structural Classification of Proteins)
        - Domain d3nuc__, region - [rasmolscript] [script source]
  • Fold representative 3nuc from FSSP and Dali (Families of Structurally Similar Proteins)
  • Domain Definition for 3nuc from 3Dee ( The Database of Protein Domain Definitions )
  • Class (fold), Architecture (subfold), Topology, Homologous superfamily: CATH
  • Summaries and structural analyses of PDB data files: PDBSum
    • Sequence-derived information
  • View one-letter amino acid or nucleotide sequence for each chain: [3nuc_]
  • SWISS-PROT database: [NUC_STAAU]
  • Domain organization of [NUC_STAAU] by SWISSPFAM
  • Domain found in 3NUC: [SNc ] by SMART
  • Conserved protein region description, domain view and alignment from family SNase (PF00565) of Pfam.
  • Alignments of the sequence of 3NUC with the sequences similar proteins can be viewed for classification [NUC_STAAU] at ProtoMap. Click on "Neighbors List", then on the "See Alignments" button below the list.
  • A sequence distance tree ("phylogenetic tree") can be viewed for 1ACL's classification [NUC_STAAU] at ProtoMap. Click on the Cluster number.
    • Other resources with information on 3NUC
  • InterPro: IPR002071 , IPR000080
  • PDBREPORT (protein verification by WHAT_CHECK procedures)
  • MMDB (Entrez's Structure Database)
    • Movements, Movies and Images
  • Images from IMB Jena Image Library of Biological Macromolecules.

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