OCA Atlas for 1MHE
1MHE Major Histocompatibility Complex date Aug 24, 1998
title The Human Non-Classical Major Histocompatibility Complex Molecule Hla-E
authors C.A.O'Callaghan, J.Tormo, B.E.Willcox, V.M.Braud, B.K.Jakobsen, D.I.Stuart, A.J.Mcmichael, J.I.Bell, E.Y.Jones
compound source
Molecule: Hla Class I Histocompatibility Antigen Hla-E
Chain: A, C
Fragment: Extracellular Domain, Alpha Chain E
Synonym: Mhc Nonclassical Chain, Mhc-E, Hla-E, Mhc Class Ib, Mhc Class 1b;
Engineered: Yes
 Organism_scientific: Homo Sapiens
Organism_common: Human
Plasmid: Coc057
Gene: Hla-E
Expression_system: Escherichia Coli
Expression_system_strain: Bl21 (De3) Plyss
Expression_system_cellular_location: Inclusion Bodies
Expression_system_plasmid: Coc057
Other_details: Denatured And Refolded In The Presence Of Peptide And Beta-2-Microglobulin;

Molecule: Beta-2-Microglobulin
Chain: B, D
Engineered: Yes
Organism_scientific: Homo Sapiens
Organism_common: Human
Plasmid: Phn1
Gene: Beta-2-Microglobulin
Expression_system: Escherichia Coli
Expression_system_strain: Xa 90
Expression_system_cellular_location: Inclusion Bodies
Expression_system_plasmid: Phn1

Molecule: Peptide (Vmaprtvll)
Chain: P, Q
Engineered: Yes
Other_details: Peptide Sequence Is That Of The Leader Sequence Of The Classical Class I Molecules
Synthetic: Yes
Organism_scientific: Homo Sapiens
Organism_common: Human
Other_details: Synthesised Chemically
symmetry Space Group: P 31 2 1
R_factor 0.202
crystal
cell
length a length b length c angle alpha angle beta angle gamma
182.200 182.200 88.400 90.00 90.00 120.00
method X-Ray Diffractionresolution 2.85 Å
ligand SO4enzyme
similarity Contains 1 immunoglobulin-like domain.[MHC_I]
subunit Dimer of alpha chain and a beta chain (beta-2- microglobulin).
subcellular loc. Secreted.
genes B2M, HLA-6.2, HLA-E, HLAE (H. sapiens)
function Beta-2-microglobulin is the beta-chain of major histocompatibility complex class i molecules. Preferably binds to a peptide derived from the signal sequence of most hla-a, -b, -c and -g molecules.
disease Hemodialysis-related amyloidosis
Data retrieval
  • Asymmetric unit, PDB entry: [header only] [complete with coordinates] (177 Kb) [Save to disk]
  • LPC: Ligand-Protein Contacts for 1MHE
  • CSU: Contacts of Structural Units for 1MHE
  • Likely Quarternary Molecular Structure file(s) for 1MHE
  • Retrieve 1MHE in mmCIF format [Save to disk]
    • View 1MHE in 3D
  • Protein Explorer, Easier to use and more powerful than RasMol. (Win and Mac only)
  • FirstGlance in Chemscape Chime (Install Chime freeware) This is good for easiest viewing of basic structure.
  • RasMol (Install RasMol freeware) Here's help on how to use RasMol.
    • Visual 3D analysis of 1MHE
  • STING, shows the sequence and maps the locations of sequence residues or residue ranges onto the 3D structure.
  • Noncovalent Bond Finder displays the closest contacts to ligand or interface, reports distances, walks over water bridges.
    • Structure-derived information
  • Dipole moment, from Dipole Server at Weizmann Institute
  • Classification of representative domains in scop (Structural Classification of Proteins)
        - Domain d1mhea1, region A:182-274 [rasmolscript] [script source]
        - Domain d1mhea2, region A:2-181 [rasmolscript] [script source]
        - Domain d1mheb1, region B: [rasmolscript] [script source]
        - Domain d1mhec1, region C:182-274 [rasmolscript] [script source]
        - Domain d1mhec2, region C:2-181 [rasmolscript] [script source]
        - Domain d1mhed1, region D: [rasmolscript] [script source]
  • Fold representative 1mhe from FSSP and Dali (Families of Structurally Similar Proteins)
  • Class (fold), Architecture (subfold), Topology, Homologous superfamily: CATH
  • Summaries and structural analyses of PDB data files: PDBSum
    • Sequence-derived information
  • View one-letter amino acid or nucleotide sequence for each chain: [1mhe_A] [1mhe_B] [1mhe_C] [1mhe_D] [1mhe_P] [1mhe_Q]
  • SWISS-PROT database: [B2MG_HUMAN] [HLAE_HUMAN]
  • Domain organization of [B2MG_HUMAN] [HLAE_HUMAN] by SWISSPFAM
  • Domain found in 1MHE: [IGc1 ] by SMART
  • Conserved protein region description, domain view and alignment from family MHC_I (PF00129) of Pfam.
  • Alignments of the sequence of 1MHE with the sequences similar proteins can be viewed for classification [B2MG_HUMAN] [HLAE_HUMAN] at ProtoMap. Click on "Neighbors List", then on the "See Alignments" button below the list.
  • A sequence distance tree ("phylogenetic tree") can be viewed for 1ACL's classification [B2MG_HUMAN] [HLAE_HUMAN] at ProtoMap. Click on the Cluster number.
    • Other resources with information on 1MHE
  • InterPro: IPR003006 , IPR001039 , IPR003597
  • PDBREPORT (protein verification by WHAT_CHECK procedures)
  • MMDB (Entrez's Structure Database)
    • Movements, Movies and Images
  • Images from IMB Jena Image Library of Biological Macromolecules.

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