Glycosylation is a post-translational modification event, which plays an important role in protein folding, cell-cell interaction and cell reorganization. Presently, more than 70% therapeutic proteins in clinical trials are glycosylated. Thus there is a need to understand and predict glycosylated residues in eukaryotic glycoproteins.


Fig. Eukaryotic glycosylated site with glycan moiety (PDB 1ZT4)

GlycoEP:is a web tool for the prediction of glycosylation sites from amino acid sequence. This server allows users to submission of multiple sequences for prediction of eukaryotic (N, O & C). Glycosites server allows users to predict glycosylation sites using both type datasets (balanced and realistic datasets).It takes amino acid sequence in FASTA format as input and predict the glycosylation sites. The amino acid residues in the query sequence predicted as Glycosylated residues are colored in red. Below the glycosylation types, the amino acid sequence and residue-wise detail prediction is also given in tabular format. The prediction result depends on the threshold value specified by the user. The default threshold is set as 0.0. To get prediction with less number of false positives, the user should choose higher threshold. For prediction with less number of false negatives, threshold should be very low.