Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_006722424                                                           17    4312
XP_005258217                                                           17    4312
NP_003271                                                              16    5631
NP_003310                                                              16    5631
NP_597676                                                              16    5631
NP_001243779                                                           16    5631
NP_596869                                                              16    5631
XP_005246888                                                           16    5631
XP_006712788                                                           16    5631
XP_006712787                                                           16    5631
XP_006712786                                                           16    5631
NP_001254479                                                           16    5631
NP_597681                                                              16    5631
NP_005804                                                              16    7355
NP_001073276                                                           16    7355
NP_001073275                                                           16    7355
NP_055039                                                              16    7355
NP_443179                                                              16    7355
XP_005276461                                                           16    7355
XP_005266703                                                           16    7355
XP_005264294                                                           16    7355
NP_008832                                                              15    9605
NP_003250                                                              15    9605
XP_005254461                                                           15    9605
XP_006720603                                                           15    9605
XP_006720602                                                           15    9605
>XP_006722424
          Length = 504

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   QATRETVTI 9
           +ATR TVTI
Sbjct: 462 EATRLTVTI 470
>XP_005258217
          Length = 504

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   QATRETVTI 9
           +ATR TVTI
Sbjct: 462 EATRLTVTI 470
>NP_003271
          Length = 161

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QATRETVT 8
           QAT ET+T
Sbjct: 122 QATGETIT 129
>NP_003310
          Length = 26926

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3     TRETVTI 9
             TRET+T+
Sbjct: 21370 TRETITL 21376
>NP_597676
          Length = 27051

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3     TRETVTI 9
             TRET+T+
Sbjct: 21495 TRETITL 21501
>NP_001243779
          Length = 34350

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3     TRETVTI 9
             TRET+T+
Sbjct: 28794 TRETITL 28800
>NP_596869
          Length = 33423

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3     TRETVTI 9
             TRET+T+
Sbjct: 27867 TRETITL 27873
>XP_005246888
          Length = 22663

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3     TRETVTI 9
             TRET+T+
Sbjct: 17107 TRETITL 17113
>XP_006712788
          Length = 24022

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3     TRETVTI 9
             TRET+T+
Sbjct: 18466 TRETITL 18472
>XP_006712787
          Length = 33978

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3     TRETVTI 9
             TRET+T+
Sbjct: 28422 TRETITL 28428
>XP_006712786
          Length = 35484

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3     TRETVTI 9
             TRET+T+
Sbjct: 29928 TRETITL 29934
>NP_001254479
          Length = 35991

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3     TRETVTI 9
             TRET+T+
Sbjct: 30435 TRETITL 30441
>NP_597681
          Length = 27118

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3     TRETVTI 9
             TRET+T+
Sbjct: 21562 TRETITL 21568
>NP_005804
          Length = 890

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  TRETVTI 9
          TRE+VTI
Sbjct: 69 TRESVTI 75
>NP_001073276
          Length = 388

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QATRETVTI 9
           Q+T ETV+I
Sbjct: 127 QSTSETVSI 135
>NP_001073275
          Length = 388

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QATRETVTI 9
           Q+T ETV+I
Sbjct: 127 QSTSETVSI 135
>NP_055039
          Length = 388

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QATRETVTI 9
           Q+T ETV+I
Sbjct: 127 QSTSETVSI 135
>NP_443179
          Length = 2170

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    QATRET 6
            QATRET
Sbjct: 1000 QATRET 1005
>XP_005276461
          Length = 388

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QATRETVTI 9
           Q+T ETV+I
Sbjct: 127 QSTSETVSI 135
>XP_005266703
          Length = 1501

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   QATRET 6
           QATRET
Sbjct: 331 QATRET 336
>XP_005264294
          Length = 890

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  TRETVTI 9
          TRE+VTI
Sbjct: 69 TRESVTI 75
>NP_008832
          Length = 2548

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TRETVTI 9
           TR+TVT+
Sbjct: 472 TRKTVTV 478
>NP_003250
          Length = 924

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TRETVTI 9
           TRE VTI
Sbjct: 313 TRENVTI 319
>XP_005254461
          Length = 2600

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TRETVTI 9
           TR+TVT+
Sbjct: 453 TRKTVTV 459
>XP_006720603
          Length = 2619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TRETVTI 9
           TR+TVT+
Sbjct: 472 TRKTVTV 478
>XP_006720602
          Length = 2619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TRETVTI 9
           TR+TVT+
Sbjct: 472 TRKTVTV 478
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.309    0.114    0.268 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 322,285
Number of extensions: 455
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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