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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000360                                                              18    1135
XP_005268094                                                           18    1135
NP_064578                                                              16    7355
NP_065961                                                              16    7355
NP_001158137                                                           16    7355
XP_006718701                                                           16    7355
XP_005274169                                                           16    7355
XP_005274166                                                           16    7355
XP_005274165                                                           16    7355
XP_005274163                                                           16    7355
XP_006716134                                                           16    7355

>NP_000360
          Length = 764

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LLEVSQTSV 9
           LL+VSQTSV
Sbjct: 230 LLDVSQTSV 238



>XP_005268094
          Length = 764

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LLEVSQTSV 9
           LL+VSQTSV
Sbjct: 230 LLDVSQTSV 238



>NP_064578
          Length = 1322

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLEVSQTSV 9
           LLE+ QT+V
Sbjct: 779 LLELQQTTV 787



>NP_065961
          Length = 1934

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LEVSQTSV 9
           +E+S+TSV
Sbjct: 498 MEISETSV 505



>NP_001158137
          Length = 1950

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LEVSQTSV 9
           +E+S+TSV
Sbjct: 498 MEISETSV 505



>XP_006718701
          Length = 1309

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLEVSQTSV 9
           LLE+ QT+V
Sbjct: 780 LLELQQTTV 788



>XP_005274169
          Length = 1272

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLEVSQTSV 9
           LLE+ QT+V
Sbjct: 743 LLELQQTTV 751



>XP_005274166
          Length = 1323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLEVSQTSV 9
           LLE+ QT+V
Sbjct: 794 LLELQQTTV 802



>XP_005274165
          Length = 1323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLEVSQTSV 9
           LLE+ QT+V
Sbjct: 780 LLELQQTTV 788



>XP_005274163
          Length = 1337

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLEVSQTSV 9
           LLE+ QT+V
Sbjct: 794 LLELQQTTV 802



>XP_006716134
          Length = 1847

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LEVSQTSV 9
           +E+S+TSV
Sbjct: 422 MEISETSV 429



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.303    0.116    0.250 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 268,630
Number of extensions: 312
Number of successful extensions: 11
Number of sequences better than 10000.0: 11
Number of HSP's gapped: 11
Number of HSP's successfully gapped: 11
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)