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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_037536                                                              16    7355
NP_005017                                                              16    7355
NP_005814                                                              16    7355
NP_001170826                                                           16    7355
XP_005255091                                                           16    7355
XP_006720900                                                           16    7355
XP_006720899                                                           16    7355
XP_006710753                                                           16    7355
XP_005263530                                                           16    7355
XP_006710752                                                           16    7355
XP_006710751                                                           16    7355
XP_006710750                                                           16    7355
XP_006710749                                                           16    7355
NP_065433                                                              15    9605
NP_955781                                                              15    9605
NP_060714                                                              15    9605
NP_060547                                                              15    9605
NP_659570                                                              15    9605
NP_001093634                                                           15    9605
NP_001138422                                                           15    9605
XP_006723877                                                           15    9605
XP_006716105                                                           15    9605
XP_005250300                                                           15    9605
XP_005250299                                                           15    9605
XP_006715978                                                           15    9605
XP_006715977                                                           15    9605
XP_006715976                                                           15    9605
XP_006715975                                                           15    9605
NP_001159607                                                           15    9605
NP_001182196                                                           15    9605
NP_001093635                                                           15    9605
NP_055536                                                              15    9605
NP_055384                                                              15    9605
NP_001093636                                                           15    9605

>NP_037536
          Length = 630

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  KNAKLST 7
          KN KLST
Sbjct: 92 KNVKLST 98



>NP_005017
          Length = 1044

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KNAKLSTTK 9
          +NA LST K
Sbjct: 38 RNANLSTIK 46



>NP_005814
          Length = 622

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  KNAKLST 7
          KN KLST
Sbjct: 92 KNVKLST 98



>NP_001170826
          Length = 622

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  KNAKLST 7
          KN KLST
Sbjct: 92 KNVKLST 98



>XP_005255091
          Length = 621

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  KNAKLST 7
          KN KLST
Sbjct: 91 KNVKLST 97



>XP_006720900
          Length = 622

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  KNAKLST 7
          KN KLST
Sbjct: 92 KNVKLST 98



>XP_006720899
          Length = 622

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  KNAKLST 7
          KN KLST
Sbjct: 92 KNVKLST 98



>XP_006710753
          Length = 1015

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KNAKLSTTK 9
          +NA LST K
Sbjct: 38 RNANLSTIK 46



>XP_005263530
          Length = 1043

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KNAKLSTTK 9
          +NA LST K
Sbjct: 38 RNANLSTIK 46



>XP_006710752
          Length = 1044

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KNAKLSTTK 9
          +NA LST K
Sbjct: 38 RNANLSTIK 46



>XP_006710751
          Length = 1044

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KNAKLSTTK 9
          +NA LST K
Sbjct: 38 RNANLSTIK 46



>XP_006710750
          Length = 1044

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KNAKLSTTK 9
          +NA LST K
Sbjct: 38 RNANLSTIK 46



>XP_006710749
          Length = 1044

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KNAKLSTTK 9
          +NA LST K
Sbjct: 38 RNANLSTIK 46



>NP_065433
          Length = 1091

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KNAKLSTT 8
           +N K+STT
Sbjct: 519 ENGKISTT 526



>NP_955781
          Length = 273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  KLSTTK 9
          KLSTTK
Sbjct: 75 KLSTTK 80



>NP_060714
          Length = 299

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  KLSTTK 9
          KLSTTK
Sbjct: 75 KLSTTK 80



>NP_060547
          Length = 759

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   AKLSTTK 9
           AKL TTK
Sbjct: 424 AKLQTTK 430



>NP_659570
          Length = 839

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   KNAKLSTTK 9
           KN KLS  K
Sbjct: 466 KNTKLSVKK 474



>NP_001093634
          Length = 645

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KNAKLSTTK 9
           +NAK  TTK
Sbjct: 407 ENAKCFTTK 415



>NP_001138422
          Length = 1573

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KNAKLSTTK 9
           KNAK  TT+
Sbjct: 634 KNAKRPTTQ 642



>XP_006723877
          Length = 220

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  KLSTTK 9
          KLSTTK
Sbjct: 75 KLSTTK 80



>XP_006716105
          Length = 734

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   AKLSTTK 9
           AKL TTK
Sbjct: 424 AKLQTTK 430



>XP_005250300
          Length = 575

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   KNAKLSTTK 9
           KN KLS  K
Sbjct: 202 KNTKLSVKK 210



>XP_005250299
          Length = 766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   KNAKLSTTK 9
           KN KLS  K
Sbjct: 393 KNTKLSVKK 401



>XP_006715978
          Length = 766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   KNAKLSTTK 9
           KN KLS  K
Sbjct: 393 KNTKLSVKK 401



>XP_006715977
          Length = 838

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   KNAKLSTTK 9
           KN KLS  K
Sbjct: 465 KNTKLSVKK 473



>XP_006715976
          Length = 839

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   KNAKLSTTK 9
           KN KLS  K
Sbjct: 466 KNTKLSVKK 474



>XP_006715975
          Length = 839

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   KNAKLSTTK 9
           KN KLS  K
Sbjct: 466 KNTKLSVKK 474



>NP_001159607
          Length = 618

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   AKLSTTK 9
           AKL TTK
Sbjct: 283 AKLQTTK 289



>NP_001182196
          Length = 951

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KNAKLSTTK 9
           KNAK  TT+
Sbjct: 634 KNAKRPTTQ 642



>NP_001093635
          Length = 565

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KNAKLSTTK 9
           +NAK  TTK
Sbjct: 327 ENAKCFTTK 335



>NP_055536
          Length = 634

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KNAKLSTTK 9
           +NAK  TTK
Sbjct: 396 ENAKCFTTK 404



>NP_055384
          Length = 839

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   KNAKLSTTK 9
           KN KLS  K
Sbjct: 466 KNTKLSVKK 474



>NP_001093636
          Length = 554

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KNAKLSTTK 9
           +NAK  TTK
Sbjct: 316 ENAKCFTTK 324



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.287   0.0993    0.226 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 275,116
Number of extensions: 250
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.9 bits)
S2: 28 (15.4 bits)