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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055365                                                              18    1482
NP_055524                                                              17    4312
NP_001258765                                                           17    4312
NP_001156795                                                           17    4312
NP_296375                                                              17    4312
NP_001156794                                                           17    4312
NP_001156793                                                           17    4312
NP_001258764                                                           17    4312
NP_001258547                                                           16    7355
NP_060077                                                              16    7355
XP_005259070                                                           16    7355
NP_060719                                                              15    9605
NP_001258968                                                           15    9605
NP_001011649                                                           15    9605
XP_006717248                                                           15    9605
XP_006717247                                                           15    9605
XP_006717246                                                           15    9605
XP_006717245                                                           15    9605
XP_006717244                                                           15    9605
XP_006717243                                                           15    9605
XP_006717242                                                           15    9605

>NP_055365
          Length = 1032

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ITMRLAQLS 9
           I MRLAQLS
Sbjct: 209 IAMRLAQLS 217



>NP_055524
          Length = 3546

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ITMRLAQLS 9
           +TMRLA LS
Sbjct: 327 LTMRLAGLS 335



>NP_001258765
          Length = 630

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   MRLAQLS 9
           MRLAQLS
Sbjct: 199 MRLAQLS 205



>NP_001156795
          Length = 618

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   MRLAQLS 9
           MRLAQLS
Sbjct: 187 MRLAQLS 193



>NP_296375
          Length = 630

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   MRLAQLS 9
           MRLAQLS
Sbjct: 199 MRLAQLS 205



>NP_001156794
          Length = 649

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   MRLAQLS 9
           MRLAQLS
Sbjct: 187 MRLAQLS 193



>NP_001156793
          Length = 696

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   MRLAQLS 9
           MRLAQLS
Sbjct: 234 MRLAQLS 240



>NP_001258764
          Length = 630

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   MRLAQLS 9
           MRLAQLS
Sbjct: 199 MRLAQLS 205



>NP_001258547
          Length = 780

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TMRLAQL 8
           T+RLAQL
Sbjct: 703 TLRLAQL 709



>NP_060077
          Length = 782

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TMRLAQL 8
           T+RLAQL
Sbjct: 705 TLRLAQL 711



>XP_005259070
          Length = 781

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TMRLAQL 8
           T+RLAQL
Sbjct: 704 TLRLAQL 710



>NP_060719
          Length = 1893

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    TMRLAQLS 9
            TM+L QLS
Sbjct: 1842 TMKLLQLS 1849



>NP_001258968
          Length = 1663

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    TMRLAQLS 9
            TM+L QLS
Sbjct: 1612 TMKLLQLS 1619



>NP_001011649
          Length = 1814

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    TMRLAQLS 9
            TM+L QLS
Sbjct: 1763 TMKLLQLS 1770



>XP_006717248
          Length = 1664

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    TMRLAQLS 9
            TM+L QLS
Sbjct: 1613 TMKLLQLS 1620



>XP_006717247
          Length = 1670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    TMRLAQLS 9
            TM+L QLS
Sbjct: 1613 TMKLLQLS 1620



>XP_006717246
          Length = 1820

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    TMRLAQLS 9
            TM+L QLS
Sbjct: 1763 TMKLLQLS 1770



>XP_006717245
          Length = 1861

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    TMRLAQLS 9
            TM+L QLS
Sbjct: 1810 TMKLLQLS 1817



>XP_006717244
          Length = 1867

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    TMRLAQLS 9
            TM+L QLS
Sbjct: 1810 TMKLLQLS 1817



>XP_006717243
          Length = 1889

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    TMRLAQLS 9
            TM+L QLS
Sbjct: 1832 TMKLLQLS 1839



>XP_006717242
          Length = 1898

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    TMRLAQLS 9
            TM+L QLS
Sbjct: 1841 TMKLLQLS 1848



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.132    0.308 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 208,264
Number of extensions: 158
Number of successful extensions: 21
Number of sequences better than 10000.0: 21
Number of HSP's gapped: 21
Number of HSP's successfully gapped: 21
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)