Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000042                                                              20     299
XP_006718908                                                           20     299
XP_005271621                                                           20     299
XP_006718907                                                           20     299
XP_006718906                                                           20     299
XP_005271619                                                           20     299
XP_005271618                                                           20     299
NP_036274                                                              17    4312
XP_006720511                                                           17    4312
NP_001265281                                                           16    5631
NP_003861                                                              16    5631
NP_777582                                                              16    5631
XP_005255041                                                           16    5631
XP_005251533                                                           16    5631
XP_006716840                                                           16    5631
XP_006716589                                                           16    5631
NP_002188                                                              16    5631
NP_997191                                                              16    7355
NP_006217                                                              16    7355
NP_001152956                                                           16    7355
NP_005800                                                              16    7355
NP_005112                                                              16    7355
XP_006715881                                                           16    7355
XP_005246701                                                           16    7355
XP_005246700                                                           16    7355
XP_006711682                                                           16    7355
NP_001073291                                                           15    9605
NP_000417                                                              15    9605
NP_065115                                                              15    9605
NP_001186704                                                           15    9605
NP_001295                                                              15    9605
NP_067064                                                              15    9605
NP_001008781                                                           15    9605
XP_005260068                                                           15    9605
XP_005260065                                                           15    9605
XP_005260064                                                           15    9605
XP_005260063                                                           15    9605
XP_005260062                                                           15    9605
XP_005260061                                                           15    9605
XP_006719457                                                           15    9605
XP_006718827                                                           15    9605
XP_005267039                                                           15    9605
XP_005267038                                                           15    9605
>NP_000042
          Length = 3056

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    DIVDGMGI 8
            DIVDGMGI
Sbjct: 2913 DIVDGMGI 2920
>XP_006718908
          Length = 1708

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    DIVDGMGI 8
            DIVDGMGI
Sbjct: 1565 DIVDGMGI 1572
>XP_005271621
          Length = 2708

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    DIVDGMGI 8
            DIVDGMGI
Sbjct: 2565 DIVDGMGI 2572
>XP_006718907
          Length = 3050

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    DIVDGMGI 8
            DIVDGMGI
Sbjct: 2907 DIVDGMGI 2914
>XP_006718906
          Length = 3056

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    DIVDGMGI 8
            DIVDGMGI
Sbjct: 2913 DIVDGMGI 2920
>XP_005271619
          Length = 3056

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    DIVDGMGI 8
            DIVDGMGI
Sbjct: 2913 DIVDGMGI 2920
>XP_005271618
          Length = 3056

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    DIVDGMGI 8
            DIVDGMGI
Sbjct: 2913 DIVDGMGI 2920
>NP_036274
          Length = 360

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   DIVDGM 6
           DIVDGM
Sbjct: 124 DIVDGM 129
>XP_006720511
          Length = 404

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   DIVDGM 6
           DIVDGM
Sbjct: 168 DIVDGM 173
>NP_001265281
          Length = 889

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   IVDGMGIM 9
           ++DG+GI+
Sbjct: 210 MIDGLGIL 217
>NP_003861
          Length = 1657

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  DIVDGMGI 8
          D VDG+G+
Sbjct: 5  DEVDGLGV 12
>NP_777582
          Length = 580

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  IVDGMG 7
          +VDGMG
Sbjct: 80 VVDGMG 85
>XP_005255041
          Length = 1191

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  DIVDGMGI 8
          D VDG+G+
Sbjct: 5  DEVDGLGV 12
>XP_005251533
          Length = 889

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   IVDGMGIM 9
           ++DG+GI+
Sbjct: 210 MIDGLGIL 217
>XP_006716840
          Length = 897

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   IVDGMGIM 9
           ++DG+GI+
Sbjct: 218 MIDGLGIL 225
>XP_006716589
          Length = 568

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  IVDGMG 7
          +VDGMG
Sbjct: 80 VVDGMG 85
>NP_002188
          Length = 889

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   IVDGMGIM 9
           ++DG+GI+
Sbjct: 210 MIDGLGIL 217
>NP_997191
          Length = 1888

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    DIVDGMGI 8
            DIV G+G+
Sbjct: 1522 DIVTGVGV 1529
>NP_006217
          Length = 1095

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   VDGMGIM 9
           VDG GIM
Sbjct: 253 VDGNGIM 259
>NP_001152956
          Length = 1736

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    DIVDGMGI 8
            DIV G+G+
Sbjct: 1522 DIVTGVGV 1529
>NP_005800
          Length = 198

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IVDGMGIM 9
           I+DG G++
Sbjct: 131 IIDGKGVL 138
>NP_005112
          Length = 2174

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    DIVDGMGI 8
            D  DGMGI
Sbjct: 1990 DGADGMGI 1997
>XP_006715881
          Length = 223

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IVDGMGI 8
           I+DG GI
Sbjct: 141 IIDGQGI 147
>XP_005246701
          Length = 1016

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   VDGMGIM 9
           VDG GIM
Sbjct: 174 VDGNGIM 180
>XP_005246700
          Length = 1021

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   VDGMGIM 9
           VDG GIM
Sbjct: 179 VDGNGIM 185
>XP_006711682
          Length = 1739

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    DIVDGMGI 8
            DIV G+G+
Sbjct: 1373 DIVTGVGV 1380
>NP_001073291
          Length = 3118

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    VDGMGI 8
            +DGMGI
Sbjct: 2981 MDGMGI 2986
>NP_000417
          Length = 3122

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    VDGMGI 8
            +DGMGI
Sbjct: 2985 MDGMGI 2990
>NP_065115
          Length = 352

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   DIVDGMG 7
           D++DG G
Sbjct: 221 DLIDGKG 227
>NP_001186704
          Length = 1133

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   DIVDGMGIM 9
           D  DG GI+
Sbjct: 555 DATDGRGIL 563
>NP_001295
          Length = 1380

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   DIVDGMGIM 9
           D  DG GI+
Sbjct: 802 DATDGRGIL 810
>NP_067064
          Length = 794

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  DIVDGMG 7
          DI+DG G
Sbjct: 59 DILDGRG 65
>NP_001008781
          Length = 4557

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    IVDGMG 7
            IVDG+G
Sbjct: 4252 IVDGLG 4257
>XP_005260068
          Length = 962

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  DIVDGMG 7
          DI+DG G
Sbjct: 59 DILDGRG 65
>XP_005260065
          Length = 1152

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  DIVDGMG 7
          DI+DG G
Sbjct: 59 DILDGRG 65
>XP_005260064
          Length = 1699

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  DIVDGMG 7
          DI+DG G
Sbjct: 59 DILDGRG 65
>XP_005260063
          Length = 1756

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  DIVDGMG 7
          DI+DG G
Sbjct: 59 DILDGRG 65
>XP_005260062
          Length = 1832

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  DIVDGMG 7
          DI+DG G
Sbjct: 59 DILDGRG 65
>XP_005260061
          Length = 1889

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  DIVDGMG 7
          DI+DG G
Sbjct: 59 DILDGRG 65
>XP_006719457
          Length = 315

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   DIVDGMG 7
           D++DG G
Sbjct: 184 DLIDGKG 190
>XP_006718827
          Length = 3479

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    IVDGMG 7
            IVDG+G
Sbjct: 3142 IVDGLG 3147
>XP_005267039
          Length = 3206

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    VDGMGI 8
            +DGMGI
Sbjct: 3069 MDGMGI 3074
>XP_005267038
          Length = 3210

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    VDGMGI 8
            +DGMGI
Sbjct: 3073 MDGMGI 3078
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.330    0.157    0.458 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 637,323
Number of extensions: 986
Number of successful extensions: 43
Number of sequences better than 10000.0: 43
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 43
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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