2PLC | Hydrolase | date | Jul 09, 1997 ![]() ![]() |
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title | Phosphatidylinositol-Specific Phospholipase C From Listeria Monocytogenes | ||||||||||||||
authors | D.W.Heinz, J.Moser | ||||||||||||||
compound | source | ||||||||||||||
Molecule: Phosphatidylinositol-Specific Phospholipase C Chain: A Synonym: Pi-Plc Ec: 3.1.4.10 Engineered: Yes |
Organism_scientific: Listeria Monocytogenes Strain: Egd Serotype 1-2a Cell_line: Bl21 Cellular_location: Secreted Into Medium Gene: Plca Expression_system: Escherichia Coli Expression_system_strain: Bl21 (De3) Expression_system_cellular_location: Cytoplasm Expression_system_vector_type: Plasmid Expression_system_plasmid: Plca Expression_system_gene: Plca Deletion Variant | ||||||||||||||
symmetry | Space Group: P 31 2 1 | R_factor | 0.198 | ||||||||||||
crystal cell |
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method | X-Ray Diffraction | resolution | 2.00 Å | ||||||||||||
ligand | enzyme | Lyase E.C.4.6.1.13 BRENDA | |||||||||||||
note | 2PLC is a representative structure | ||||||||||||||
related structures | by homologous chain: 1AOD | ||||||||||||||
similarity | Contains 1 pi-plc x-box domain.[PI-PLC-X] | ||||||||||||||
subunit | Monomer. | ||||||||||||||
catalytic activ. | 1-phosphatidyl-1d-myo-inositol = 1d-myo- inositol 1,2-cyclic phosphate + diacylglycerol. | ||||||||||||||
subcellular loc. |
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Genes | LMO0201, PIC, PLCA (L. monocytogenes) | ||||||||||||||
function | Important factor in pathogenesis, pi-plc activity is present only in virulent listeria species. It may participate in the lysis of the phagolysosomal membrane. Cleaves glycosylphosphatidylinositol (gpi) and phosphatidylinositol (pi) anchors but not pi phosphates. | ||||||||||||||
Gene Ontology |
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Primary reference | Crystal structure of the phosphatidylinositol-specific phospholipase C from the human pathogen Listeria monocytogenes., Moser J, Gerstel B, Meyer JE, Chakraborty T, Wehland J, Heinz DW, J Mol Biol 1997 Oct 17;273(1):269-82. PMID:9367761 |
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Structure-derived information |
- Domain d2plc__, region [Jmol] [rasmolscript] [script source] |
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