2PLC	Hydrolase	date	Jul 09, 1997
title	Phosphatidylinositol-Specific Phospholipase C From Listeria Monocytogenes
authors	D.W.Heinz, J.Moser
compound		source
Molecule: Phosphatidylinositol-Specific Phospholipase C Chain: A Synonym: Pi-Plc Ec: 3.1.4.10 Engineered: Yes		Organism_scientific: Listeria Monocytogenes Strain: Egd Serotype 1-2a Cell_line: Bl21 Cellular_location: Secreted Into Medium Gene: Plca Expression_system: Escherichia Coli Expression_system_strain: Bl21 (De3) Expression_system_cellular_location: Cytoplasm Expression_system_vector_type: Plasmid Expression_system_plasmid: Plca Expression_system_gene: Plca Deletion Variant
symmetry	Space Group: P 31 2 1	R_factor	0.198
crystal cell	length a length b length c angle alpha angle beta angle gamma 82.500 82.500 92.200 90.00 90.00 120.00
method	X-Ray Diffraction	resolution	2.00 Å
ligand		enzyme	Lyase E.C.4.6.1.13 BRENDA
note	2PLC is a representative structure
related structures	by homologous chain: 1AOD
similarity	Contains 1 pi-plc x-box domain.[PI-PLC-X]
subunit	Monomer.
catalytic activ.	1-phosphatidyl-1d-myo-inositol = 1d-myo- inositol 1,2-cyclic phosphate + diacylglycerol.
subcellular loc.	Membrane localization by OPM: Secreted out side in side
Genes	LMO0201, PIC, PLCA (L. monocytogenes)
function	Important factor in pathogenesis, pi-plc activity is present only in virulent listeria species. It may participate in the lysis of the phagolysosomal membrane. Cleaves glycosylphosphatidylinositol (gpi) and phosphatidylinositol (pi) anchors but not pi phosphates.
Gene Ontology	Chain Function Process Component A phospholipase C activity signal transduction intracellular signaling casc...
Primary reference	Crystal structure of the phosphatidylinositol-specific phospholipase C from the human pathogen Listeria monocytogenes., Moser J, Gerstel B, Meyer JE, Chakraborty T, Wehland J, Heinz DW, J Mol Biol 1997 Oct 17;273(1):269-82. PMID:9367761

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (50 Kb) [Save to disk] Biological Unit Coordinates (2plc.pdb1.gz) 47 Kb CSU: Contacts of Structural Units for 2PLC Likely Quarternary Molecular Structure file(s) for 2PLC Structure Factors (598 Kb) Retrieve 2PLC in mmCIF format [Save to disk]

View 2PLC in 3D

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 2PLC, from MSDmotif at EBI Genome occurence of 2PLC's fold from GeneCensus Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d2plc__, region [Jmol] [rasmolscript] [script source] Fold representative 2plc from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [2plc_A]

Other resources with information on 2PLC

InterPro: IPR000909 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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