2J0N	Cell Invasion	date	Aug 03, 2006
title	A Proteolytically Truncated Form Of Shigella Flexneri Ipad
authors	S.Johnson, P.Roversi, S.M.Lea
compound		source
Molecule: Invasin Ipad Chain: A, B Fragment: Truncated Form, Residues 133-332 Synonym: Ipad Engineered: Yes Other_details: The Truncation Was Obtained By In-Drop Cleavage By Unkown Protease Of A Construct Consisting Of Residues 15-332		Organism_scientific: Shigella Flexneri Organism_common: Shigella Strain: 301 Variant: Serotype 2a Atcc: 700930 Expression_system: Escherichia Coli Expression_system_strain: Bl21 Expression_system_variant: De3 Expression_system_plasmid: Pet-15b
symmetry	Space Group: C 1 2 1	R_factor	0.189
crystal cell	length a length b length c angle alpha angle beta angle gamma 77.790 91.360 54.910 90.00 96.35 90.00
method	X-Ray Diffraction	resolution	2.10 Å
Genes	CP0126, IPAD (S. flexneri)
Gene Ontology	Chain Function Process Component A pathogenesis
Primary reference	Self-chaperoning of the type III secretion system needle tip proteins IpaD and BipD., Johnson S, Roversi P, Espina M, Olive A, Deane JE, Birket S, Field T, Picking WD, Blocker AJ, Galyov EE, Picking WL, Lea SM, J Biol Chem. 2007 Feb 9;282(6):4035-44. Epub 2006 Oct 31. PMID:17077085

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (65 Kb) [Save to disk] Biological Unit Coordinates (2j0n.pdb1.gz) 61 Kb CSU: Contacts of Structural Units for 2J0N Likely Quarternary Molecular Structure file(s) for 2J0N Structure Factors (224 Kb) Retrieve 2J0N in mmCIF format [Save to disk]

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 2J0N, from MSDmotif at EBI Fold representative 2j0n from FSSP and Dali (Families of Structurally Similar Proteins)

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [2j0n_A] [2j0n_B]

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