2I5Z	De Novo Protein	date	Aug 26, 2006
title	The Crystal Structure Of Ospa Mutant
authors	K.Makabe, V.Terechko, S.Koide
compound		source
Molecule: Outer Surface Protein A Chain: O Engineered: Yes		Organism_scientific: Borrelia Burgdorferi Organism_common: Lyme Disease Spirochete Gene: Ospa Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Bl21(De3) Expression_system_vector_type: Plasmid Expression_system_plasmid: Pet24a
symmetry	Space Group: P 1 21 1	R_factor	0.148
crystal cell	length a length b length c angle alpha angle beta angle gamma 34.783 53.840 65.495 90.00 99.32 90.00
method	X-Ray Diffraction	resolution	1.20 Å
ligand	PG4	enzyme
Gene	OSPA (B. burgdorferi)
Gene Ontology	Chain Function Process Component O cell outer membrane membrane outer membrane
Primary reference	Hydrophobic surface burial is the major stability determinant of a flat, single-layer beta-sheet., Yan S, Gawlak G, Makabe K, Tereshko V, Koide A, Koide S, J Mol Biol. 2007 Apr 20;368(1):230-43. Epub 2007 Feb 7. PMID:17335845

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (101 Kb) [Save to disk] Biological Unit Coordinates (2i5z.pdb1.gz) 98 Kb CSU: Contacts of Structural Units for 2I5Z Structure Factors (659 Kb) Retrieve 2I5Z in mmCIF format [Save to disk]

View 2I5Z in 3D

Proteopedia, because life has more than 2D. On Jmol, a nice Rasmol like molecule viewer. This is good for easiest viewing of basic structure. On FirstGlance, an excellent tool for a guided tour on the structure components, by E. Martz. On AstexViewer, from MSD-EBI (viewer documentation). On RasMol (Install RasMol freeware) Here's help on how to use RasMol.

Visual 3D analysis of 2I5Z

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 2I5Z, from MSDmotif at EBI Fold representative 2i5z from FSSP and Dali (Families of Structurally Similar Proteins)

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [2i5z_O]

Other resources with information on 2I5Z

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