2GDC	Cell Invasion	date	Mar 15, 2006
title	Structure Of Vinculin Vd1 Ipaa560-633 Complex
authors	C.Hamiaux, A.Van Eerde, C.Parsot, J.Broos, B.W.Dijkstra
compound		source
Molecule: Vinculin Chain: A Fragment: Vd1 Domain (Residues 0-265) Synonym: Metavinculin Engineered: Yes		Organism_scientific: Gallus Gallus Organism_common: Chicken Gene: Vcl, Vinc1 Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Rosetta2 Expression_system_vector_type: Plasmid Expression_system_plasmid: Pmal-P2
Molecule: Invasin Ipaa Chain: B Fragment: C-Terminal Fragment (Residues 560-633) Synonym: 70 Kda Antigen Engineered: Yes		Organism_scientific: Shigella Flexneri Organism_common: Bacteria Gene: Ipaa Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Dh5alpha Expression_system_vector_type: Plasmid Expression_system_plasmid: Pgex4t2
symmetry	Space Group: P 21 21 2	R_factor	0.247
crystal cell	length a length b length c angle alpha angle beta angle gamma 52.684 68.326 96.521 90.00 90.00 90.00
method	X-Ray Diffraction	resolution	2.74 Å
Genes	CP0125, IPAA (S. flexneri); VCL, VINC1 (G. gallus)
Gene Ontology	Chain Function Process Component A actin binding structural molecule activity... protein binding oxidoreductase activity cell adhesion metabolic process lamellipodium biogenesis regulation of cell migration... cytoplasm cytoskeleton intercellular junction adherens junction focal adhesion actin cytoskeleton cell junction costamere B actin binding vinculin binding pathogenesis positive regulation of actin...
Primary reference	Structural mimicry for vinculin activation by IpaA, a virulence factor of Shigella flexneri., Hamiaux C, van Eerde A, Parsot C, Broos J, Dijkstra BW, EMBO Rep. 2006 Aug;7(8):794-9. Epub 2006 Jul 7. PMID:16826238

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (45 Kb) [Save to disk] Biological Unit Coordinates (2gdc.pdb1.gz) 41 Kb CSU: Contacts of Structural Units for 2GDC Likely Quarternary Molecular Structure file(s) for 2GDC Structure Factors (238 Kb) Retrieve 2GDC in mmCIF format [Save to disk]

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Structure-derived information

Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 2GDC, from MSDmotif at EBI Fold representative 2gdc from FSSP and Dali (Families of Structurally Similar Proteins)

Sequence-derived information

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