2FK4	Metal Binding Protein, Oncoprotein	date	Jan 04, 2006
title	Solution Structure Of The C-Terminal Zinc Binding Domain Of The Hpv16 E6 Oncoprotein
authors	Y.Nomine, S.Charbonnier
compound		source
Molecule: Protein E6 Chain: A Fragment: C-Terminal Domain Engineered: Yes Mutation: Yes		Organism_scientific: Human Papillomavirus Type 16 Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Bl21 De2 Expression_system_vector_type: Plasmid
method	NMR, 10 Structures
ligand	ZN	enzyme
note	2FK4 is a representative structure
similarity	Belongs to the papillomaviruses E6 protein family.
subunit	Forms a complex with e6-ap ubiquitin-protein ligase which interacts with human p53. Binds to human fbln1 and mpdz (by similarity).
subcellular loc.	Nuclear matrix-associated.
Gene	E6 (H. papillomavirus)
function	Interaction with human fbln1 protein also seems to be linked to cell transformation. Those two functions presumably contribute to transforming activity (by similarity). Transcriptional transactivator. Has transforming activity. Inactivates, with e6-ap ubiquitin- protein ligase, the human tp53/p53 tumor suppressor protein by targeting it to degradation. Binds and targets human mupp1/mpdz protein to degradation. Binds double stranded dna.
Gene Ontology	Chain Function Process Component A DNA binding protein binding zinc ion binding metal ion binding transcription regulation of transcription,... nucleus host cell nucleus
Primary reference	Structural and functional analysis of E6 oncoprotein: insights in the molecular pathways of human papillomavirus-mediated pathogenesis., Nomine Y, Masson M, Charbonnier S, Zanier K, Ristriani T, Deryckere F, Sibler AP, Desplancq D, Atkinson RA, Weiss E, Orfanoudakis G, Kieffer B, Trave G, Mol Cell. 2006 Mar 3;21(5):665-78. PMID:16507364

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (213 Kb) [Save to disk] Biological Unit Coordinates (2fk4.pdb1.gz) 211 Kb CSU: Contacts of Structural Units for 2FK4 Original NMR restraints for 2FK4 from PDB Retrieve 2FK4 in mmCIF format [Save to disk]

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Structure-derived information

Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 2FK4, from MSDmotif at EBI Fold representative 2fk4 from FSSP and Dali (Families of Structurally Similar Proteins)

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [2fk4_A]

Other resources with information on 2FK4

InterPro: IPR001334 MMDB (Entrez's Structure Database)

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