2EPH	Lyase	date	Mar 30, 2007
title	Crystal Structure Of Fructose-Bisphosphate Aldolase From Plasmodium Falciparum In Complex With Trap-Tail Determined At 2.7 Angstrom Resolution
authors	J.Bosch, C.A.Buscaglia, B.Krumm, T.Cardozo, V.Nussenzweig, W.G.J.Hol, Structural Genomics Of Pathogenic Protozoa Consortium (Sgpp)
compound		source
Molecule: Fructose-Bisphosphate Aldolase Chain: A, B, C, D Synonym: 41 Kda Antigen Ec: 4.1.2.13 Engineered: Yes		Organism_scientific: Plasmodium Falciparum Organism_common: Malaria Parasite Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Bl21star(De3) Expression_system_vector_type: Plasmid Expression_system_vector: T7 System Expression_system_plasmid: Pet14b
Molecule: Pbtrap Chain: H Fragment: C-Terminus: Residues 601-606 Engineered: Yes		Synthetic: Yes Other_details: Synthetic Peptide With The Sequence Based On Pbtrap Protein From Plasmodium Berghei, Unp Entry P90573, P90573_plabe, Residues 601-606
symmetry	Space Group: P 21 21 21	R_factor	0.192
crystal cell	length a length b length c angle alpha angle beta angle gamma 70.019 146.162 148.961 90.00 90.00 90.00
method	X-Ray Diffraction	resolution	2.70 Å
ligand		enzyme	Lyase E.C.4.1.2.13 BRENDA
Gene	PBTRAP (P. berghei)
Gene Ontology	Chain Function Process Component A, D, C, B catalytic activity fructose-bisphosphate aldola... lyase activity glycolysis metabolic process
Primary reference	Aldolase provides an unusual binding site for thrombospondin-related anonymous protein in the invasion machinery of the malaria parasite., Bosch J, Buscaglia CA, Krumm B, Ingason BP, Lucas R, Roach C, Cardozo T, Nussenzweig V, Hol WG, Proc Natl Acad Sci U S A. 2007 Apr 24;104(17):7015-20. Epub 2007 Apr 10. PMID:17426153

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (234 Kb) [Save to disk] Biological Unit Coordinates (2eph.pdb1.gz) 224 Kb CSU: Contacts of Structural Units for 2EPH Likely Quarternary Molecular Structure file(s) for 2EPH Structure Factors (1763 Kb) Retrieve 2EPH in mmCIF format [Save to disk]

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 2EPH, from MSDmotif at EBI Fold representative 2eph from FSSP and Dali (Families of Structurally Similar Proteins)

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [2eph_A] [2eph_D] [2eph_B] [2eph_H] [2eph_C]

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