2CHB | Toxin | date | Jun 03, 1997 ![]() |
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title | Cholera Toxin B-Pentamer Complexed With Gm1 Pentasaccharide | ||||||||||||||
authors | E.A.Merritt, W.G.J.Hol | ||||||||||||||
compound | source | ||||||||||||||
Molecule: Cholera Toxin Chain: D, E, F, G, H Fragment: B-Pentamer Engineered: Yes Biological_unit: Pentamer Other_details: Receptor Binding Site On Each Monomer Occupied By Gm1 Pentasaccharide |
Organism_scientific: Vibrio Cholerae Strain: Ogawa 41 (Classical Biotype) Expression_system: Escherichia Coli | ||||||||||||||
symmetry | Space Group: C 1 2 1 | R_factor | 0.171 | ||||||||||||
crystal cell |
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method | X-Ray Diffraction | resolution | 2.0 Å | ||||||||||||
ligand | GAL, GLC, NGA, SIA | enzyme |
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note | 2CHB supersedes 1CHB | ||||||||||||||
similarity | Belongs to the Enterotoxin_b family. | ||||||||||||||
subunit | The a subunit contains two chains, a1 and a2, linked by a disulfide bridge. The holotoxin (choleragen) consists of a pentameric ring of b subunits whose central pore is occupied by the a subunit. | ||||||||||||||
gene | ctxB (V. cholerae) | ||||||||||||||
function | It can bind five gm1 gangliosides. The b subunit pentameric ring directs the a subunit to its target by binding to the gm1 gangliosides present on the surface of the intestinal epithelial cells. It has no toxic activity by itself. | ||||||||||||||
Gene Ontology |
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Primary reference | Structural studies of receptor binding by cholera toxin mutants., Merritt EA, Sarfaty S, Jobling MG, Chang T, Holmes RK, Hirst TR, Hol WG, Protein Sci 1997 Jul;6(7):1516-28. PMID:9232653 |
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Structure-derived information |
- Domain d2chbd_, region D [Jmol] [rasmolscript] [script source] - Domain d2chbe_, region E [Jmol] [rasmolscript] [script source] - Domain d2chbf_, region F [Jmol] [rasmolscript] [script source] - Domain d2chbg_, region G [Jmol] [rasmolscript] [script source] - Domain d2chbh_, region H [Jmol] [rasmolscript] [script source] |
Sequence-derived information |
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