2BGC	Transcription	date	Dec 16, 2004
title	Prfa-G145s, A Constitutive Active Mutant Of The Transcriptional Regulator In L.Monocytogenes
authors	M.Eiting, G.Hagelueken, W.D.Schubert, D.W.Heinz
compound		source
Molecule: Prfa Chain: A, B, D, E, F, G, H, I Engineered: Yes		Organism_scientific: Listeria Monocytogenes Strain: Egd-E Expression_system: Escherichia Coli Expression_system_strain: M15 Expression_system_plasmid: Pqe-30
symmetry	Space Group: C 1 2 1	R_factor	0.195
crystal cell	length a length b length c angle alpha angle beta angle gamma 117.247 100.243 189.563 90.00 90.33 90.00
method	X-Ray Diffraction	resolution	2.30 Å
ligand	DTT, DTU, PR3	enzyme
note	2BGC is a representative structure
related structures	by homologous chain: 2BEO
similarity	Contains1 hth crp-type dna-binding domain.
Genes	LMO0200, PRFA (L. monocytogenes)
function	Positively regulates expression of listeriolysin, of 1- phosphadidylinositol phosphodiesterase (pi-plc) and other virulence factors.
Gene Ontology	Chain Function Process Component H, F, A, D, I, G, E, B DNA binding transcription regulation of transcription,... pathogenesis regulation of transcription
Primary reference	The mutation G145S in PrfA, a key virulence regulator of Listeria monocytogenes, increases DNA-binding affinity by stabilizing the HTH motif., Eiting M, Hageluken G, Schubert WD, Heinz DW, Mol Microbiol. 2005 Apr;56(2):433-46. PMID:15813735

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (322 Kb) [Save to disk] Biological Unit Coordinates (2bgc.pdb1.gz) 88 Kb Biological Unit Coordinates (2bgc.pdb2.gz) 80 Kb Biological Unit Coordinates (2bgc.pdb3.gz) 80 Kb Biological Unit Coordinates (2bgc.pdb4.gz) 79 Kb CSU: Contacts of Structural Units for 2BGC Likely Quarternary Molecular Structure file(s) for 2BGC Structure Factors (735 Kb) Retrieve 2BGC in mmCIF format [Save to disk]

View 2BGC in 3D

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Visual 3D analysis of 2BGC

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Structure-derived information

Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 2BGC, from MSDmotif at EBI Fold representative 2bgc from FSSP and Dali (Families of Structurally Similar Proteins)

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [2bgc_H] [2bgc_G] [2bgc_D] [2bgc_A] [2bgc_F] [2bgc_E] [2bgc_B] [2bgc_I]

Other resources with information on 2BGC

InterPro: IPR012318 , IPR000595 , IPR011991 , IPR001808 PDBREPORT (protein verification by WHAT_CHECK procedures) MMDB (Entrez's Structure Database)

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