1ZID	Oxidoreductase	date	Mar 25, 1997
title	Long Fatty Acid Chain Enoyl-Acp Reductase (Inha) In Complex With An Isonicotinic-Acyl-Nadh Inhibitor
authors	D.A.Rozwarski, W.R.Jacobs Jr., J.C.Sacchettini, Tb Structural Genomics Consortium (Tbsgc)
compound		source
Molecule: Enoyl-[Acyl-Carrier-Protein] Reductase Chain: Null Synonym: Inha, Enoyl-Acp Reductase Ec: 1.3.1.9 Engineered: Yes Mutation: T2a Biological_unit: Homotetramer		Organism_scientific: Mycobacterium Tuberculosis Organism_common: Tb Expression_system: Escherichia Coli Expression_system_strain: Bl21
symmetry	Space Group: P 62 2 2	R_factor	0.202
crystal cell	length a length b length c angle alpha angle beta angle gamma 100.530 100.530 138.960 90.00 90.00 120.00
method	X-Ray Diffraction	resolution	2.7 Å
ligand	ZID	enzyme	Enoyl-[acyl-carrier-protein] reductase (NADH);. Enoyl-[acyl carrier protein] reductase;. Enoyl-ACP reductase;. NADH-enoyl acyl carrier protein reductase;. NADH-specific enoyl-ACP reductase;. Enoyl-[acyl-carrier-protein] reductase (NADH2). Oxidoreductase E.C.1.3.1.9 BRENDA
similarity	Fabisubfamily. Belongs to the short-chain dehydrogenases/reductases (sdr) family.
subunit	Homotetramer.
catalytic activ.	Acyl-[acyl-carrier protein] + nad(+) = trans- 2,3-dehydroacyl-[acyl-carrier protein] + nadh.
pathway	Second reductive step in fatty acid biosynthesis. This isozyme is involved in mycolic acid biosynthesis.
genes	fabI (E. coli); fabI (H. pylori); MT1531, inhA (M. tuberculosis)
function	Involved in the resistance against the antituberculosis drugs isoniazid and ethionamide.
Gene Ontology	Chain Function Process Component A enoyl-[acyl-carrier-protein]... oxidoreductase activity fatty acid biosynthetic proc... metabolic process lipid biosynthetic process response to antibiotic
Primary reference	Modification of the NADH of the isoniazid target (InhA) from Mycobacterium tuberculosis., Rozwarski DA, Grant GA, Barton DH, Jacobs WR Jr, Sacchettini JC, Science 1998 Jan 2;279(5347):98-102. PMID:9417034

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (54 Kb) [Save to disk] Biological Unit Coordinates (1zid.pdb1.gz) 165 Kb CSU: Contacts of Structural Units for 1ZID Likely Quarternary Molecular Structure file(s) for 1ZID Structure Factors (74 Kb) Retrieve 1ZID in mmCIF format [Save to disk]

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1ZID, from MSDmotif at EBI Genome occurence of 1ZID's fold from GeneCensus Classification of representative domains in scop (Structural Classification of Proteins)     - Domain d1zid__, region [Jmol] [rasmolscript] [script source] Fold representative 1zid from FSSP and Dali (Families of Structurally Similar Proteins) Class (fold), Architecture (subfold), Topology, Homologous superfamily from CATH Summaries and structural analyses of PDB data files from PDBSum Identification of Protein Pockets & Cavities at CASTp

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1zid_]

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