1XN1	Transferase	date	Oct 04, 2004
title	Crystal Structure Of Lumazine Synthase From Brucella Abortus (Orthorhombic Form At 3.05 Angstroms)
authors	S.Klinke, V.Zylberman, D.R.Vega, B.G.Guimaraes, B.C.Braden, F.A.Goldbaum
compound		source
Molecule: 6,7-Dimethyl-8-Ribityllumazine Synthase Chain: A, B, C, D, E, F, G, H, I, J Synonym: Dmrl Synthase, Lumazine Synthase, Riboflavin Synthase Beta Chain; Ec: 2.5.1.9 Engineered: Yes		Organism_scientific: Brucella Abortus Organism_common: Bacteria Gene: Ribh Expression_system: Escherichia Coli Expression_system_common: Bacteria Expression_system_strain: Bl21(De3) Expression_system_vector_type: Plasmid Expression_system_plasmid: Pet11b
symmetry	Space Group: P 21 21 21	R_factor	0.199
crystal cell	length a length b length c angle alpha angle beta angle gamma 116.554 120.238 159.372 90.00 90.00 90.00
method	X-Ray Diffraction	resolution	3.05 Å
ligand	NA, PO4, SO4	enzyme	Transferase E.C.2.5.1.9 BRENDA
Genes	BRUAB2 ; RIBH-2, RIBH2, RIBH (B. abortus)
Gene Ontology	Chain Function Process Component F, A, J, E, B, H, C, D, I, G riboflavin synthase activity... transferase activity riboflavin biosynthetic proc... riboflavin synthase complex
Primary reference	Crystallographic Studies on Decameric Brucella spp. Lumazine Synthase: A Novel Quaternary Arrangement Evolved for a New Function?, Klinke S, Zylberman V, Vega DR, Guimaraes BG, Braden BC, Goldbaum FA, J Mol Biol 2005 Oct 14;353(1):124-37. PMID:16165152

Data retrieval

Asymmetric unit, PDB entry: [header only] [complete with coordinates] (235 Kb) [Save to disk] Biological Unit Coordinates (1xn1.pdb1.gz) 231 Kb CSU: Contacts of Structural Units for 1XN1 Likely Quarternary Molecular Structure file(s) for 1XN1 Structure Factors (353 Kb) Retrieve 1XN1 in mmCIF format [Save to disk]

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Structure-derived information

Electron Density related parameters from EDS Electron Density Server, at Upsala Dipole moment, from Dipole Server at Weizmann Institute Crystal Contacts, from CryCo at Weizmann Institute 3D motif for 1XN1, from MSDmotif at EBI Fold representative 1xn1 from FSSP and Dali (Families of Structurally Similar Proteins)

Sequence-derived information

View one-letter amino acid or nucleotide sequence for each chain: [1xn1_E] [1xn1_J] [1xn1_G] [1xn1_H] [1xn1_B] [1xn1_F] [1xn1_C] [1xn1_A] [1xn1_D] [1xn1_I]

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